4gec: Difference between revisions
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{{STRUCTURE_4gec| PDB=4gec | SCENE= }} | |||
===Crystal Structure of E.coli MenH R124A Mutant=== | |||
==Function== | |||
[[http://www.uniprot.org/uniprot/MENH_ECOLI MENH_ECOLI]] Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also able to catalyze the hydrolysis of the thioester bond in palmitoyl-CoA in vitro.<ref>PMID:15808744</ref> <ref>PMID:18284213</ref> | |||
==About this Structure== | |||
[[4gec]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GEC OCA]. | |||
==Reference== | |||
<references group="xtra"/><references/> | |||
[[Category: 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase]] | |||
[[Category: Escherichia coli k-12]] | |||
[[Category: Baker, E N.]] | |||
[[Category: Guo, Z.]] | |||
[[Category: Jiang, M.]] | |||
[[Category: Johnston, J M]] | |||
[[Category: 2-succinyl-6-hydroxy-2]] | |||
[[Category: 4-cyclohexadiene-1-carboxylate synthase]] | |||
[[Category: Alpha beta hydrolase]] | |||
[[Category: Lyase]] | |||
[[Category: Menaquinone biosynthesis]] | |||
[[Category: Menh mutant r124a]] | |||
[[Category: Mutant r124a]] | |||
Revision as of 07:52, 8 May 2013
Crystal Structure of E.coli MenH R124A Mutant
Function
[MENH_ECOLI] Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also able to catalyze the hydrolysis of the thioester bond in palmitoyl-CoA in vitro.[1] [2]
About this Structure
4gec is a 3 chain structure with sequence from Escherichia coli k-12. Full crystallographic information is available from OCA.
Reference
- ↑ Kuznetsova E, Proudfoot M, Sanders SA, Reinking J, Savchenko A, Arrowsmith CH, Edwards AM, Yakunin AF. Enzyme genomics: Application of general enzymatic screens to discover new enzymes. FEMS Microbiol Rev. 2005 Apr;29(2):263-79. PMID:15808744 doi:S0168-6445(05)00004-5
- ↑ Jiang M, Chen X, Guo ZF, Cao Y, Chen M, Guo Z. Identification and characterization of (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase in the menaquinone biosynthesis of Escherichia coli. Biochemistry. 2008 Mar 18;47(11):3426-34. doi: 10.1021/bi7023755. Epub 2008 Feb, 20. PMID:18284213 doi:10.1021/bi7023755
