3tgn: Difference between revisions

Line 1:

[[~~Image~~:~~3tgn~~.~~png~~|~~left~~|~~200px~~]]

==Crystal Structure of the zinc-dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-bound State==

== Structural highlights ==

<table><tr><td colspan='2'>[[3tgn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TGN FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>

<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">adcR, SPD_2000 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])</td></tr>

<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tgn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tgn RCSB], [http://www.ebi.ac.uk/pdbsum/3tgn PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

Streptococcus pneumoniae adhesin competence regulator (AdcR), the first metal-dependent member of the multiple antibiotic resistance regulator (MarR) family of proteins, represses the transcription of a high-affinity zinc-specific uptake transporter, a group of surface antigen zinc-binding pneumococcal histidine triad proteins (PhtA, PhtB, PhtD, and PhtE), and an AdcA homologue (AdcAII). The 2.0 A resolution structure of Zn(II)-bound AdcR reveals a highly helical two-fold-symmetric dimer with two distinct metal-binding sites per protomer. Zn(II) is tetrahedrally coordinated by E24, H42, H108, and H112 in what defines the primary sensing site in AdcR. Site 2 is a tetracoordinate site whose function is currently unknown. NMR methyl group perturbation experiments reveal that Zn(II) drives a global change in the structure of apo-AdcR that stabilizes a conformation that is compatible with DNA binding. This co-repression mechanism is unprecedented in MarR transcriptional regulators.

~~<!--~~

Crystal Structure of the Zinc-Dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-Bound State.,Guerra AJ, Dann CE, Giedroc DP J Am Chem Soc. 2011 Nov 21. PMID:22085181<ref>PMID:22085181</ref>

~~The line below this paragraph, containing "STRUCTURE_3tgn", creates the "~~Structure ~~Box" on~~ the ~~page.~~

~~You may change~~ the ~~PDB parameter~~ (~~which sets the PDB file loaded into the applet)~~

~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded~~),

~~or leave the SCENE parameter empty for the default display~~.

-->

~~{{STRUCTURE_3tgn| PDB=3tgn | SCENE= }}~~

~~===Crystal Structure~~ of the ~~zinc-dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-bound State===~~

From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>

</div>

== References ==

~~<!--~~

~~The line below this paragraph, {{ABSTRACT_PUBMED_22085181}}, adds the Publication Abstract to the page~~

__TOC__

~~(as it appears on PubMed at http://www~~.~~pubmed~~.~~gov), where 22085181 is the PubMed ID number~~.

</StructureSection>

-->

~~{{ABSTRACT_PUBMED_22085181}}~~

~~==About this Structure==~~

~~[[3tgn]] is a 2 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TGN OCA].~~

==~~Reference~~==

<~~ref group="xtra">PMID:022085181<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Streptococcus pneumoniae]]

[[Category: Giedroc, D P.]]

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@@ Line 1: / Line 1: @@
-[[Image:3tgn.png|left|200px]]
+==Crystal Structure of the zinc-dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-bound State==
+<StructureSection load='3tgn' size='340' side='right' caption='[[3tgn]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[3tgn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TGN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TGN FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
+<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">adcR, SPD_2000 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1313 Streptococcus pneumoniae])</td></tr>
+<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tgn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tgn RCSB], [http://www.ebi.ac.uk/pdbsum/3tgn PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Streptococcus pneumoniae adhesin competence regulator (AdcR), the first metal-dependent member of the multiple antibiotic resistance regulator (MarR) family of proteins, represses the transcription of a high-affinity zinc-specific uptake transporter, a group of surface antigen zinc-binding pneumococcal histidine triad proteins (PhtA, PhtB, PhtD, and PhtE), and an AdcA homologue (AdcAII). The 2.0 A resolution structure of Zn(II)-bound AdcR reveals a highly helical two-fold-symmetric dimer with two distinct metal-binding sites per protomer. Zn(II) is tetrahedrally coordinated by E24, H42, H108, and H112 in what defines the primary sensing site in AdcR. Site 2 is a tetracoordinate site whose function is currently unknown. NMR methyl group perturbation experiments reveal that Zn(II) drives a global change in the structure of apo-AdcR that stabilizes a conformation that is compatible with DNA binding. This co-repression mechanism is unprecedented in MarR transcriptional regulators.
-<!--
+Crystal Structure of the Zinc-Dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-Bound State.,Guerra AJ, Dann CE, Giedroc DP J Am Chem Soc. 2011 Nov 21. PMID:22085181<ref>PMID:22085181</ref>
-The line below this paragraph, containing "STRUCTURE_3tgn", creates the "Structure Box" on the page.
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-or leave the SCENE parameter empty for the default display.
--->
-{{STRUCTURE_3tgn|  PDB=3tgn  |  SCENE=  }}
-===Crystal Structure of the zinc-dependent MarR Family Transcriptional Regulator AdcR in the Zn(II)-bound State===
+From MEDLINEÂ®/PubMedÂ®, a database of the U.S. National Library of Medicine.<br>
+</div>
+== References ==
-<!--
+<references/>
-The line below this paragraph, {{ABSTRACT_PUBMED_22085181}}, adds the Publication Abstract to the page
+__TOC__
-(as it appears on PubMed at http://www.pubmed.gov), where 22085181 is the PubMed ID number.
+</StructureSection>
--->
-{{ABSTRACT_PUBMED_22085181}}
-==About this Structure==
-[[3tgn]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_pneumoniae Streptococcus pneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TGN OCA].
-==Reference==
-<ref group="xtra">PMID:022085181</ref><references group="xtra"/>
 [[Category: Streptococcus pneumoniae]]
 [[Category: Giedroc, D P.]]