4ibn: Difference between revisions
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==Crystal structure of LC9-RNase H1, a type 1 RNase H with the type 2 active-site motif== | |||
<StructureSection load='4ibn' size='340' side='right' caption='[[4ibn]], [[Resolution|resolution]] 1.62Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4ibn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Uncultured_organism Uncultured organism]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4IBN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4IBN FirstGlance]. <br> | |||
</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_H Ribonuclease H], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.4 3.1.26.4] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ibn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ibn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ibn RCSB], [http://www.ebi.ac.uk/pdbsum/4ibn PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from alphaA-helix to beta1-strand immediately next to the first aspartate residue, as in the active site of RNase H2. However, the structure and enzymatic properties of LC9-RNase H1 highly resemble those of RNase H1, instead of RNase H2. We propose that LC9-RNase H1 represents bacterial RNases H1 with an atypical DEDN active site motif, which are evolutionarily distinct from those with a typical DEDD active site motif. | |||
Crystal structure of metagenome-derived LC9-RNase H1 with atypical DEDN active site motif.,Nguyen TN, You DJ, Kanaya E, Koga Y, Kanaya S FEBS Lett. 2013 May 2;587(9):1418-23. doi: 10.1016/j.febslet.2013.03.020. Epub, 2013 Mar 20. PMID:23523920<ref>PMID:23523920</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
==See Also== | |||
*[[Ribonuclease|Ribonuclease]] | |||
*[[Temp|Temp]] | |||
*[[User:Jaime.Prilusky/Test/tree|User:Jaime.Prilusky/Test/tree]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Ribonuclease H]] | [[Category: Ribonuclease H]] | ||
[[Category: Uncultured organism]] | [[Category: Uncultured organism]] | ||
[[Category: Kanaya, E | [[Category: Kanaya, E]] | ||
[[Category: Kanaya, S | [[Category: Kanaya, S]] | ||
[[Category: Koga, Y | [[Category: Koga, Y]] | ||
[[Category: Nguyen, T N | [[Category: Nguyen, T N]] | ||
[[Category: You, D J | [[Category: You, D J]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Hydrolases']] | [[Category: Hydrolases']] | ||
[[Category: Ribonuclease h]] | [[Category: Ribonuclease h]] | ||
[[Category: Rnase h]] | [[Category: Rnase h]] | ||
Revision as of 12:16, 21 December 2014
Crystal structure of LC9-RNase H1, a type 1 RNase H with the type 2 active-site motif
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