4na2: Difference between revisions
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==Crystal Structure of the second ketosynthase from the bacillaene polyketide synthase bound to its natural intermediate== | |||
<StructureSection load='4na2' size='340' side='right' caption='[[4na2]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
{ | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4na2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NA2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4NA2 FirstGlance]. <br> | |||
==Function== | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=2JF:(2S)-2-AMINO-3-OXOPROPYL+4-{[(2S)-2-HYDROXY-4-METHYLPENTANOYL]AMINO}BUTANOATE'>2JF</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2qo3|2qo3]], [[2hg4|2hg4]], [[4na1|4na1]], [[4na3|4na3]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">pksJ, pksK, BSU17180 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4na2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4na2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4na2 RCSB], [http://www.ebi.ac.uk/pdbsum/4na2 PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/PKSJ_BACSU PKSJ_BACSU]] Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.<ref>PMID:16460000</ref> <ref>PMID:17234808</ref> | [[http://www.uniprot.org/uniprot/PKSJ_BACSU PKSJ_BACSU]] Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism.<ref>PMID:16460000</ref> <ref>PMID:17234808</ref> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The recently discovered trans-acyltransferase modular polyketide synthases catalyze the biosynthesis of a wide range of bioactive natural products in bacteria. Here we report the structure of the second ketosynthase from the bacillaene trans-acyltransferase polyketide synthase. This 1.95 A resolution structure provides the highest resolution view available of a modular polyketide synthase ketosynthase and reveals a flanking subdomain that is homologous to an ordered linker in cis-acyltransferase modular polyketide synthases. The structure of the cysteine-to-serine mutant of the ketosynthase acylated by its natural substrate provides high-resolution details of how a native polyketide intermediate is bound and helps explain the basis of ketosynthase substrate specificity. The substrate range of the ketosynthase was further investigated by mass spectrometry. | |||
A Close Look at a Ketosynthase from a Trans-Acyltransferase Modular Polyketide Synthase.,Gay DC, Gay G, Axelrod AJ, Jenner M, Kohlhaas C, Kampa A, Oldham NJ, Piel J, Keatinge-Clay AT Structure. 2014 Feb 4. pii: S0969-2126(14)00008-2. doi:, 10.1016/j.str.2013.12.016. PMID:24508341<ref>PMID:24508341</ref> | |||
== | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | |||
[[Category: Gay, D C | == References == | ||
[[Category: Gay, G R | <references/> | ||
[[Category: Keatinge-Clay, A T | __TOC__ | ||
</StructureSection> | |||
[[Category: Bacsu]] | |||
[[Category: Gay, D C]] | |||
[[Category: Gay, G R]] | |||
[[Category: Keatinge-Clay, A T]] | |||
[[Category: Condensing enzyme fold]] | [[Category: Condensing enzyme fold]] | ||
[[Category: Transferase]] | [[Category: Transferase]] | ||
Revision as of 20:45, 25 December 2014
Crystal Structure of the second ketosynthase from the bacillaene polyketide synthase bound to its natural intermediate
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