4zbl: Difference between revisions
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zbl OCA], [http://pdbe.org/4zbl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zbl RCSB], [http://www.ebi.ac.uk/pdbsum/4zbl PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4zbl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zbl OCA], [http://pdbe.org/4zbl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4zbl RCSB], [http://www.ebi.ac.uk/pdbsum/4zbl PDBsum]</span></td></tr> | ||
</table> | </table> | ||
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== Publication Abstract from PubMed == | |||
Phototoxic fluorescent proteins represent a sparse group of genetically encoded photosensitizers that could be used for precise light-induced inactivation of target proteins, DNA damage, and cell killing. Only two such GFP-based fluorescent proteins (FPs), KillerRed and its monomeric variant SuperNova, were described up to date. Here, we present a crystallographic study of their two orange successors, dimeric KillerOrange and monomeric mKillerOrange, at 1.81 and 1.57 A resolution, respectively. They are the first orange-emitting protein photosensitizers with a tryptophan-based chromophore (Gln65-Trp66-Gly67). Same as their red progenitors, both orange photosensitizers have a water-filled channel connecting the chromophore to the beta-barrel exterior and enabling transport of ROS. In both proteins, Trp66 of the chromophore adopts an unusual trans-cis conformation stabilized by H-bond with the nearby Gln159. This trans-cis conformation along with the water channel was shown to be a key structural feature providing bright orange emission and phototoxicity of both examined orange photosensitizers. | |||
Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore.,Pletneva NV, Pletnev VZ, Sarkisyan KS, Gorbachev DA, Egorov ES, Mishin AS, Lukyanov KA, Dauter Z, Pletnev S PLoS One. 2015 Dec 23;10(12):e0145740. doi: 10.1371/journal.pone.0145740., eCollection 2015. PMID:26699366<ref>PMID:26699366</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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<div class="pdbe-citations 4zbl" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
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</StructureSection> | </StructureSection> | ||
Revision as of 12:29, 13 January 2016
Phototoxic fluorescent protein mKillerOrange
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