Rho GTPase

spinqualitylabelsall models Human RhoA (magenta) complex with RhoGAP (cyan), GDP, AlF4 and Mg+2 ion (green) (PDB code 1tx4) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Rho GTPase of higher vertebrates include RhoA, RhoB, RhoC, RhoD and RhoE. These 3 proteins share 85% sequence identity. RhoA is the more extensively studied among these three. RhoA regulates a signal transduction phosphorylation pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Rho GTPase operates as a switch cycling between the active GTP-bound form and the inactive GDP-bound one. A number of proteins have been identified as targets of RhoA. Mitochondrial Rho GTPase (Miro) have tandem GTP-binding domains separated by a linker region containing calcium-binding EF hand motifs indicating a role in mitochondrial homeostasis and apoptosis. Disease Mutations in RhoA were identified in diffuse gastric cancer. Relevance RhoA is thought to be important in mediating vasoconstriction in the penis. Structural highlights The transition state complex of RhoA and RhoGAP contains GDP, AlF4 and Mg+2 ion. AlF4 and Mg+2 ion binding site.

3D structures of Rho GTPase

Updated on 25-October-2017

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• RhoA
  • 5ez6, 5c4m, 5a0f, 1dpf, 1ftn – hRhoA + GDP – human
    
  • 5a0f - hRhoA (mutant) + GDP
    
  • 1kmq – hRhoA (mutant) + GNP
    
  • 1a2b – hRhoA + GSP
    
  • 3tvd – mRhoA + GSP – mouse
    
• RhoA complex with guanine nucleotide exchange factor
  • 4xh9 – hRhoA (mutant) + guanine nucleotide exchange factor NET1
    
  • 5jhg, 5jhh - hRhoA + guanine nucleotide exchange factor 11
    
  • 3t06, 1xcg – hRhoA (mutant) + guanine nucleotide exchange factor 11
    
  • 1lb1 – hRhoA (mutant) + guanine nucleotide exchange factor Dbs
    
  • 3kz1 – hRhoA + guanine nucleotide exchange factor 11 + GSP
    
  • 1x86 – hRhoA + guanine nucleotide exchange factor 12
    
• RhoA complex with Rho GTPase-activating protein
  • 5irc – hRhoA + Rho GTPase-activating protein 20 + GDP
    
  • 1ow3, 5m6x, 5m70 – hRhoA + Rho GTPase-activating protein 1 + GDP
    
  • 3msx – hRhoA (mutant) + Rho GTPase-activating protein 35 + GDP
    
• RhoA complex with ADP-ribosyltransferase
  • 4xsg – hRhoA (mutant) + ADP-ribosyltransferase + GTP
    
  • 4xsh – hRhoA (mutant) + ADP-ribosyltransferase + GSP + NADH
    
  • 5bwm – hRhoA (mutant) + ADP-ribosyltransferase + GDP + NADH
    
• RhoA other complexes
  • 5fr2, 5fr1, 1cc0 – hRhoA + Rho GDP-dissociation inhibitor 1 + GDP
    
  • 5hpy – hRhoA (mutant) + unconventional myosin-IXB + GDP
    
  • 4xoi – hRhoA (mutant) + actin-binding protein anillin + GTP
    
  • 4f38 – mRhoA + Rho GDP-dissociation inhibitor 1 + GPPNP
    
  • 4d0n – hRhoA + A-kinase anchor protein 13 + GDP
    
  • 3lw8, 3lwn, 3lxr – hRhoA + IpgB2 + GDP
    
  • 1s1c – hRhoA + ROCKI + GNP
    
  • 1cxz – hRhoA + Pkn/Prk1 + GSP
    
  • 1tx4 – hRhoA + RhoGap + GDP
    
  • 2rgn – hRhoA + guanine nucleotide-binding protein alpha-Q + RhoA/Rac/CDC42 exchange factor + GDP
    
  • 5c2k – hRhoA/Rac GTPase-activating protein 1 + GDP
    
  • 5jcp – hRhoA/ARAP3 + GDP
    
• RhoB
  • 2fv8 – hRhoB + GDP
    
• RhoC
  • 2gcn – hRhoC + GDP
    
  • 2gco – hRhoC + GPPNP
    
  • 2gcp – hRhoC + GSP
    
  • 2gcn – hRhoC (mutant) + diaphanous protein homolog 1 + GMPPNP
    
• RhoD
  • 2j1l – hRhoD + GDP
    
• RhoE
  • 1m7b – hRhoE + GTP
    
  • 1gwn – mRhoE core domain + GTP
    
  • 2v55 – hRhoE + ROCKI + GTP + ANP
    
• Miro1
  • 5kso, 5ksp, 5ksy – hMiro1 C-terminal + GDP
    
  • 5ksz – hMiro1 EF hand and kinase domain + GMPPCP
    
  • 5kty, 5ku1 – hMiro1 EF hand and kinase domain + GDP
    
• Miro2
  • 5kut – hMiro2 C-terminal + GDP
    
• Miro
  • 4c0j, 4c0k – DmMiro – Drosophila melanogaster
    
  • 4c0l – DmMiro + GDP
    

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