3w9v

Crystal structure of refolded DING protein

spinqualitylabelsall models 3w9v, resolution 1.03Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3w9v is a 2 chain structure with sequence from Unkp. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GOL, PO4 Related: 2v3q, 3g62, 2q9t, 3w9w Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Disease [PHBP_UNKP] May be involved in atherosclerosis. Function [PHBP_UNKP] Phosphate-binding protein. Publication Abstract from PubMed After crystallization of a certain protein-RNA complex, well diffracting crystals were obtained. However, the asymmetric unit of the crystal was too small to locate any components. Mass spectrometry and X-ray crystal structure analysis showed that it was a member of the DING protein family (HPBP). Surprisingly, the structure of HPBP reported previously was also determined accidentally as a contaminant, suggesting that HPBP has a strong tendency to crystallize. Furthermore, DING proteins were reported to relate in disease. These observations suggest that DING has potential for application in a wide range of research fields. To enable further analyses, a system for preparation of HPBP was constructed. As HPBP was expressed in insoluble form in Escherichia coli, it was unfolded chemically and refolded. Finally, a very high yield preparation method was constructed, in which 43 mg of HPBP was obtained from 1 L of culture. Furthermore, to evaluate the validity of refolding, its crystal structure was determined at 1.03 A resolution. The determined structure was identical to the native structure, in which two disulfide bonds were recovered correctly and a phosphate ion was captured. Based on these results, it was concluded that the refolded HPBP recovers its structure correctly. Crystal structure analysis, overexpression and refolding behaviour of a DING protein with single mutation.,Gai Z, Nakamura A, Tanaka Y, Hirano N, Tanaka I, Yao M J Synchrotron Radiat. 2013 Nov;20(Pt 6):854-8. doi: 10.1107/S0909049513020694., Epub 2013 Sep 29. PMID:24121327 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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