Resolvase

spinqualitylabelsall models Yeast Holliday junction resolvase dimer complex with sulfate, 1kcf Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Resolvase or recombinase (Rec) is a nuclease which is involved in DNA recombination. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. Tyr-recombinases include Cre which recombines loxP sites, FLP and lambda integrase (LamInt). Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. RadA recombinase promotes DNA recombination. Detailed analysis of its dimeric structure suggests mechanisms for junction isomerization and communication between the two active sites . Hin-recombinase (HRec) is a protein of Salmonella. HRec inverts a 900 base pair DNA segment which contains the promoters of flagellar genes. The inversion changes the these genes' expressions.

3D structures of resolvase

Updated on 04-September-2018

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• Holliday junction resolvase
  • 2wiw, 2wiz, 2wj0 – AfHJR+DNA – Archaeoglobus fulgidus
    
  • 2wcw, 2wcz – AfHJR (mutant)
    
  • 2h8c – EcHJR RusA (mutant) + DNA – Escherichia coli
    
  • 1gdt - EcHJR +DNA
    
  • 2h8e - EcHJR RusA (mutant)
    
  • 1q8r – EcHJR RusA
    
  • 1hjr – EcHJR RuvC
    
  • 4ep4 – TtHJR RuvC – Thermus thermophilus
    
  • 4ep5 – TtHJR RuvC (mutant)
    
  • 1zp7 – BsHJR RecU – Bacillus subtilis
    
  • 5fdk - BsHJR RecU (mutant) + DNA
    
  • 1ob8, 1ob9, 1hh1, 2eo0 – SsHJR – Sulfolobus solfataricus
    
  • 4tkd, 4tkk - SsHJR (mutant) -
    
  • 2fco – HJR – Geobacillus kaustophilus
    
  • 1ipi, 1gef – PfHJR – Pyrococcus furiosus
    
  • 1kcf – HJR – fission yeast
    
  • 1bvs - RuvA - Mycobacterium leprae
    
  • 5e6f - HJR - canarypox virus
    
• Ser-recombinase
  • 2rsl - EcGDR
    
  • 1hx7 – EcGDR N-terminal – NMR
    
  • 1ght, 1res, 1ret, 1ght – EcGDR catalytic domain – NMR
    
  • 1gdr, 1hx7 - EcGDR catalytic domain
    
  • 2gm4, 1zr2, 1zr4, 1gdt – EcGDR+DNA
    
  • 2gm5 – EcGDR (mutant)
    
  • 3guv – Sp-site-specific recombinase – Streptococcus pneumoniae
    
  • 2r0q – SaS-rec+DNA – Staphylococcus aureus
    
  • 3pkz - SaS-sin (mutant)
    
  • 3lhf – SsS-rec
    
• Tyr-recombinase
  • 3etl, 3ew9, 3ewa, 2i1q – MmRadA Rec+AMPPNP+ion – Methanococcus maripaludis
    
  • 2b21 - MmRadA Rec+AMPPNP
    
  • 2zub, 2zuc, 2zud, 2dfl – SsRadA
    
  • 2cvf, 2cvh – RadB Rec – Thermococcus kodakarensis
    
  • 2f1h, 2fpl, 2fpm - MvRec+AMPPNP+K – Methanococcus voltae
    
  • 2f1i – MvRec+AMPPNP
    
  • 2f1j, 2fpk - MvRec+ADP
    
  • 1a0p – EcXerD Rec
    
  • 5dcf - EcXerD C-terminal/FTSK
    
  • 5hxy - XerD - Thermoplasma acidophilum
    
  • 5jjv, 5jk0 - XerH - Helicobacter pylori
    
• Cre recombinase
  • 3mgv, 3c28, 3c29, 2hof, 2hoi, 1xns, 1xo0, 1pvp, 1pvq, 1pvr, 1nzb, 1ouq, 1q3u, 1q3v, 1ma7, 1kbu, 1drg, 1f44, 2crx, 3crx, 4crx, 5crx, 1crx – EpCre+DNA – Enterobacteria phage PI
    
• Hin recombinase
  • 1hcr, 1ijw, 1ijj6, 1jj8, 1jko, 1jkp, 1jkq, 1jkr – HRec + DNA – synthetic
    
• RadA recombinase
  • 2zub, 2zuc, 2zud, 2dfl – SsRadA
    
  • 4dc9 – MvRadA C-terminal domain
    
  • 2i1q – MvRadA (mutant)
    
  • 1pzn, 5fos, 5fot, 5fou, 5fox, 5fpk, 5fov, 5fow – PfRadA
    
  • 4a6p – PfRadA ATPase domain (mutant)
    
  • 5lkq - SpRadA protease domain
    
  • 5j4l, 5jec, 5jed, 5jee, 5kdd, 5l8v, 5lb2, 5lb4, 5lbi, 5jfg - PfRadA (mutant)
    
  • 5h45 - TtRadA C-terminal
    
• RadA recombinase complex
  • 5fot, 5fou, 5fov, 5fow, 5fpk, 4b3b – PfRadA ATPase domain + tetrapeptide
    
  • 4d6p – PfRadA ATPase domain (mutant) + AMPPNP
    
  • 4uqo – PfRadA ATPase domain (mutant) + ADP
    
  • 4b2i – PfRadA ATPase domain (mutant) + indazole
    
  • 4b2l, 4b35 – PfRadA ATPase domain (mutant) + methylester tryplophan
    
  • 4b32, 4b33 – PfRadA ATPase domain (mutant) + naphthol
    
  • 4b34 – PfRadA ATPase domain (mutant) + aminobenzothiazole
    
  • 4b3c, 4b3d, 5j4h, 5j4k – PfRadA ATPase domain (mutant) + indole derivative
    
  • 4a6x – PfRadA ATPase domain (mutant) + ATP
    
  • 3ntu, 2gdj – MvRadA + AMPPNP
    
  • 2b21, 2f1h, 2f1i, 2fpl, 2fpm – MvRadA (mutant) + AMPPNP
    
  • 3fyh, 2f1j, 2fpk – MvRadA (mutant) + ADP
    
  • 3etl, 3ew9, 3ewa – MmRadA (mutant) + AMPPNP
    
  • 5lkm - SpRadA + TDP
    
• RadB recombinase
  • 2cvf, 2cvh – TkRadB
    
• Integrase see Integrase and Retroviral Integrase
• Recombinase A (RecA) see Recombinase A

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Alexander Berchansky, Jaime Prilusky, Joel L. Sussman, Michal Harel