6cpv: Difference between revisions

Line 1:

~~'''Unreleased structure'''~~

~~The entry~~ 6cpv is ~~ON HOLD until Paper~~ Publication

==MicroED structure of NaK ion channel reveals a process of Na+ partition into the selectivity filter==

== Structural highlights ==

<table><tr><td colspan='2'>[[6cpv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CPV FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cpv OCA], [http://pdbe.org/6cpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cpv RCSB], [http://www.ebi.ac.uk/pdbsum/6cpv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cpv ProSAT]</span></td></tr>

</table>

== Publication Abstract from PubMed ==

Sodium (Na(+)) is a ubiquitous and important inorganic salt mediating many critical biological processes such as neuronal excitation, signaling, and facilitation of various transporters. The hydration states of Na(+) are proposed to play critical roles in determining the conductance and the selectivity of Na(+) channels, yet they are rarely captured by conventional structural biology means. Here we use the emerging cryo-electron microscopy (cryoEM) method micro-electron diffraction (MicroED) to study the structure of a prototypical tetrameric Na(+)-conducting channel, NaK, to 2.5 A resolution from nano-crystals. Two new conformations at the external site of NaK are identified, allowing us to visualize a partially hydrated Na(+) ion at the entrance of the channel pore. A process of dilation coupled with Na(+) movement is identified leading to valuable insights into the mechanism of ion conduction and gating. This study lays the ground work for future studies using MicroED in membrane protein biophysics.

~~Authors~~:

MicroED structure of the NaK ion channel reveals a Na(+) partition process into the selectivity filter.,Liu S, Gonen T Commun Biol. 2018;1:38. doi: 10.1038/s42003-018-0040-8. Epub 2018 May 3. PMID:30167468<ref>PMID:30167468</ref>

~~Description~~:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

[[Category: ~~Unreleased Structures~~]]

</div>

== References ==

__TOC__

</StructureSection>

[[Category: Gonen, T]]

[[Category: Liu, S]]

[[Category: Ion channel]]

[[Category: Nak]]

[[Category: Transport protein]]

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6cpv is ON HOLD  until Paper Publication
+==MicroED structure of NaK ion channel reveals a process of Na+ partition into the selectivity filter==
+<StructureSection load='6cpv' size='340' side='right' caption='[[6cpv]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6cpv]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CPV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CPV FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cpv OCA], [http://pdbe.org/6cpv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cpv RCSB], [http://www.ebi.ac.uk/pdbsum/6cpv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cpv ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Sodium (Na(+)) is a ubiquitous and important inorganic salt mediating many critical biological processes such as neuronal excitation, signaling, and facilitation of various transporters. The hydration states of Na(+) are proposed to play critical roles in determining the conductance and the selectivity of Na(+) channels, yet they are rarely captured by conventional structural biology means. Here we use the emerging cryo-electron microscopy (cryoEM) method micro-electron diffraction (MicroED) to study the structure of a prototypical tetrameric Na(+)-conducting channel, NaK, to 2.5 A resolution from nano-crystals. Two new conformations at the external site of NaK are identified, allowing us to visualize a partially hydrated Na(+) ion at the entrance of the channel pore. A process of dilation coupled with Na(+) movement is identified leading to valuable insights into the mechanism of ion conduction and gating. This study lays the ground work for future studies using MicroED in membrane protein biophysics.
-Authors:
+MicroED structure of the NaK ion channel reveals a Na(+) partition process into the selectivity filter.,Liu S, Gonen T Commun Biol. 2018;1:38. doi: 10.1038/s42003-018-0040-8. Epub 2018 May 3. PMID:30167468<ref>PMID:30167468</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6cpv" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Gonen, T]]
+[[Category: Liu, S]]
+[[Category: Ion channel]]
+[[Category: Nak]]
+[[Category: Transport protein]]