2ex5

Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA

spinqualitylabelsall models 2ex5, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2ex5 is a 4 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: CA Related: 1af5, 1bp7, 1g9y, 1g9z, 1m5x, 1mow Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [DNE1_CHLMO] Endonuclease involved in intron homing. Recognizes a degenerate sequence of 17-19 bp to produce a staggered cut 5 bp downstream from the CeLSU.5 intron insertion site. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the endonuclease ORF. We have determined the structure and DNA recognition behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from Chlamydomonas eugametos. This symmetric endonuclease displays unique structural elaborations on its core enzyme fold, and it preferentially cleaves a highly asymmetric target site. This latter property represents an early step, prior to gene fusion, in the generation of asymmetric DNA binding platforms from homodimeric ancestors. The divergence of the sequence, structure, and target recognition behavior of homing endonucleases, as illustrated by this study, leads to the invasion of novel genomic sites by mobile introns during evolution. The structure of I-CeuI homing endonuclease: Evolving asymmetric DNA recognition from a symmetric protein scaffold.,Spiegel PC, Chevalier B, Sussman D, Turmel M, Lemieux C, Stoddard BL Structure. 2006 May;14(5):869-80. PMID:16698548 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Endonuclease

Proteopedia Page Contributors and Editors (what is this?)

OCA