1cfr

CRYSTAL STRUCTURE OF CITROBACTER FREUNDII RESTRICTION ENDONUCLEASE CFR10I AT 2.15 ANGSTROMS RESOLUTION.

spinqualitylabelsall models 1cfr, resolution 2.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1cfr is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Activity: Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [T2CX_CITFR] Recognizes the double-stranded sequence RCCGGY and cleaves after R-1. Publication Abstract from PubMed The X-ray crystal structure of Citrobacter freundii restriction endonuclease Cfr10I has been determined at a resolution of 2.15 A by multiple isomorphous replacement methods and refined to an R-factor of 19.64%. The structure of Cfr10I represents the first structure of a restriction endonuclease recognizing a degenerated nucleotide sequence. Structural comparison of Cfr10I with previously solved structures of other restriction enzymes suggests that recognition of specific sequence occurs through contacts in the major and the minor grooves of DNA. The arrangement of the putative active site residues shows some striking differences from previously described restriction endonucleases and supports a two-metal-ion mechanism of catalysis. Crystal structure of Citrobacter freundii restriction endonuclease Cfr10I at 2.15 A resolution.,Bozic D, Grazulis S, Siksnys V, Huber R J Mol Biol. 1996 Jan 12;255(1):176-86. PMID:8568865 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Endonuclease

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