1es8

Crystal structure of free BglII

spinqualitylabelsall models 1es8, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 1es8 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: ACY NonStd Res: MSE Related: 1dfm, 1d2i Activity: Type II site-specific deoxyribonuclease, with EC number 3.1.21.4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [T2B2_BACIU] Recognizes the double-stranded sequence AGATCT and cleaves after A-1. Publication Abstract from PubMed Restriction endonuclease BglII completely encircles its target DNA, making contacts to both the major and minor grooves. To allow the DNA to enter and leave the binding cleft, the enzyme dimer has to rearrange. To understand how this occurs, we have solved the structure of the free enzyme at 2.3 A resolution, as a complement to our earlier work on the BglII-DNA complex. Unexpectedly, the enzyme opens by a dramatic 'scissor-like' motion, accompanied by a complete rearrangement of the alpha-helices at the dimer interface. Moreover, within each monomer, a set of residues--a 'lever'--lowers or raises to alternately sequester or expose the active site residues. Such an extreme difference in free versus complexed structures has not been reported for other restriction endonucleases. This elegant mechanism for capturing DNA may extend to other enzymes that encircle DNA. Structure of free BglII reveals an unprecedented scissor-like motion for opening an endonuclease.,Lukacs CM, Kucera R, Schildkraut I, Aggarwal AK Nat Struct Biol. 2001 Feb;8(2):126-30. PMID:11175900 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Endonuclease

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