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| == Structural highlights == | | == Structural highlights == |
| The <scene name='74/749425/Cv/5'>RNA loop is sandwiched between RBD 1 and 2</scene> interacting with both NCL domains<ref>PMID:15033352</ref>.<scene name='74/749425/Cv/3'>RNA-protein interactions</scene>. | | The <scene name='74/749425/Cv/6'>RNA loop is sandwiched between RBD 1 and 2</scene> interacting with both NCL domains<ref>PMID:15033352</ref>. <scene name='74/749425/Cv/7'>RNA-protein interactions</scene>. |
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| </StructureSection> | | </StructureSection> |
Revision as of 12:47, 24 July 2019
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Function
Nucleolin (NCL) is a multifunctional phosphoprotein found in the nucleolus, nucleus and cytoplasm. NCL contains intrinsic DNA and RNA helicase, nucleic-acid-dependent ATPase and self-cleaving activities. NCL binds RNA through its RNA recognition motifs (RBD). It regulates various aspects of DNA and RNA metabolism[1].
Relevance
NCL expression is enhanced on the surface of many different types of cancer cells and hence is the target of anti-cancer therapies[2]. NCL is a specific marker of angiogenic endothelial cells in tumor vessels[3].
Structural highlights
The RNA loop is sandwiched between RBD 1 and 2 interacting with both NCL domains[4]. RNA-protein interactions.
- ↑ Tajrishi MM, Tuteja R, Tuteja N. Nucleolin: The most abundant multifunctional phosphoprotein of nucleolus. Commun Integr Biol. 2011 May;4(3):267-75. doi: 10.4161/cib.4.3.14884. PMID:21980556 doi:https://dx.doi.org/10.4161/cib.4.3.14884
- ↑ Koutsioumpa M, Papadimitriou E. Cell surface nucleolin as a target for anti-cancer therapies. Recent Pat Anticancer Drug Discov. 2014 May;9(2):137-52. PMID:24251811
- ↑ Christian S, Pilch J, Akerman ME, Porkka K, Laakkonen P, Ruoslahti E. Nucleolin expressed at the cell surface is a marker of endothelial cells in angiogenic blood vessels. J Cell Biol. 2003 Nov 24;163(4):871-8. PMID:14638862 doi:https://dx.doi.org/10.1083/jcb.200304132
- ↑ Johansson C, Finger LD, Trantirek L, Mueller TD, Kim S, Laird-Offringa IA, Feigon J. Solution structure of the complex formed by the two N-terminal RNA-binding domains of nucleolin and a pre-rRNA target. J Mol Biol. 2004 Apr 2;337(4):799-816. PMID:15033352 doi:https://dx.doi.org/10.1016/j.jmb.2004.01.056
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3D structures of nucleolin
Updated on 24-July-2019
2krr – hNCL RBD1-2 – human - NMR
2fc8, 2fc9 – hNCL RRM_1 domain - NMR
1rkj, 1fje – haNCL RBD1-2 + RNA – hamster - NMR
1fj7 – haNCL RBD1 - NMR
1fjc – haNCL RBD2 - NMR
References
