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| <StructureSection load='' size='350' side='right' caption='Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry [[2gq3]])' scene='57/573146/Cv/1'> | | <StructureSection load='' size='350' side='right' caption='Structure of malate synthase G complex with CoA, malate, Hepes and Mg+2 ion (green) (PDB entry [[2gq3]])' scene='57/573146/Cv/1'> |
| == Function == | | == Function == |
| '''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to (S)-malate and CoA. MS participates in pyruvate, glyoxylate and dicarboxylate metabolism. There are 2 isozymes of MS. '''MS G''' is formed during growth on glycolate<ref>PMID:12930982</ref> and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate. | | '''Malate synthase''' (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to [[R/S nomencalture|(S)]]-malate and CoA. MS participates in pyruvate, glyoxylate and dicarboxylate metabolism. There are 2 isozymes of MS. '''MS G''' is formed during growth on glycolate<ref>PMID:12930982</ref> and '''MS A''' which metabolizes glyoxylate formed in the dissimilation of acetate. |
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| == Structural highlights == | | == Structural highlights == |
Revision as of 21:28, 29 August 2019
| Function
Malate synthase (MS) catalyzes the reversible conversion of acetyl-CoA, glyoxylate and water to (S)-malate and CoA. MS participates in pyruvate, glyoxylate and dicarboxylate metabolism. There are 2 isozymes of MS. MS G is formed during growth on glycolate[1] and MS A which metabolizes glyoxylate formed in the dissimilation of acetate.
Structural highlights
MS active site pocket is situated between the TIM barrel and the C-terminal. The ternary complex contains malate, acetyl-CoA and Mg+2 ion[2].
Malate/Mg+2 ion binding site. Water molecules are shown as red spheres.
Acetyl-CoA binding site.
- ↑ Anstrom DM, Kallio K, Remington SJ. Structure of the Escherichia coli malate synthase G:pyruvate:acetyl-coenzyme A abortive ternary complex at 1.95 A resolution. Protein Sci. 2003 Sep;12(9):1822-32. PMID:12930982
- ↑ Anstrom DM, Remington SJ. The product complex of M. tuberculosis malate synthase revisited. Protein Sci. 2006 Aug;15(8):2002-7. PMID:16877713 doi:15/8/2002
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3d structures of malate synthase
Updated on 29-August-2019
{"openlevels":0}
- Malate synthase
- (S), 2jqx – EcMS G (mutant) – Escherichia coli - NMR
- 3cuz, 3cv1 – EcMS A
- 4ex4 – MS G + Mg – Mycobacterium leprae
- 5h8p – MtMS G – Mycobacterium tuberculosis
- 5h8m – MtMS G (mutant) + Mg
- 3cux – MS + Mg – Bacillus anthracis
- 5oas – PaMS G + Mg – Pseudomonas aeruginosa
- Malate synthase binary complex
- 1d8c – EcMS G (mutant) + glyoxylate + Mg
- 3cv2 – EcMS A + oxalate + Mg
- 1n8i – MtMS G + glyoxylate + Mg
- 5h8u – MtMS G + malate + Mg
- 56axe – MtMS G + acetyl CoA + Mg
- 5c7v, 5cah, 5cjm, 5e9x – MtMS G (mutant) + pyrrole derivative + Mg
- 5cew – MtMS G (mutant) + pyridine derivative + Mg
- 5cjn – MtMS G (mutant) + quinoxaline derivative + Mg
- 5dx7 – MtMS G (mutant) + isoxazole derivative + Mg
- 5c9r, 5c9u, 5c9w, 5cbj, 5ccz – MtMS G (mutant) + phenyl derivative + Mg
- 5c9x, 5cbi – MtMS G (mutant) + benzoate derivative + Mg
- 5cak, 5cbb, 5cc3, 5cc5, 5cc6, 5cc7, 5drc, 5dri, 5ecv – MtMS G (mutant) + indole derivative + Mg
- 5t8g – MtMS G (mutant) + thiophene derivative + Mg
- 3s9i, 3s9z, 3sad, 3saz, 3sb0 – MtMS G (mutant) + inhibitor + Mg
- 3oyx, 3pug – HvMS G + glyoxylate + Mg – Haloferax volcanii
- 5tao – HvMS G + glyoxylate + Pb
- 5vfb – PaMS + glycolate + Ni
- Malate synthase ternary complex
- 1p7t – EcMS G (mutant) + pyruvate + Mg + acetyl-CoA
- 3oyz – HvMS + pyruvate + Mg + acetyl-CoA
- 1n8w – MtMS G + glyoxylate + Mg + CoA
- 2gq3 – MtMS G + malate + Mg + CoA
References
