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| == Structural highlights == | | == Structural highlights == |
| The <scene name='47/471830/Cv/5'>MBP sugar binding cleft is rich with protein-ligand hydrogen bonds</scene><ref>PMID:9309217</ref>. Water molecules are shown as red spheres. | | The <scene name='47/471830/Cv/5'>MBP sugar binding cleft is rich with protein-ligand hydrogen bonds</scene><ref>PMID:9309217</ref>. Water molecules are shown as red spheres. |
| | |
| | ==3D structures of maltose-binding protein== |
| | [[Maltose-binding protein 3D structures]] |
| | |
| </StructureSection> | | </StructureSection> |
| ==3D structures of maltose-binding protein== | | ==3D structures of maltose-binding protein== |
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| **[[4khz]] - MBP MalK, MalF, MalG, MBP <br /> | | **[[4khz]] - MBP MalK, MalF, MalG, MBP <br /> |
| **[[3fh6]] - MBP MalK, MalF, MalG <br /> | | **[[3fh6]] - MBP MalK, MalF, MalG <br /> |
| **[[2xd2]] – SpMBP – ''Streptococcus pneumoniae'' | | **[[2xd2]] – SpMBP – ''Streptococcus pneumoniae''<br /> |
| | **[[6dtt]] - TmMBP male2 – ''Thermotoga maritima''<br /> |
| | **[[6dtr]] - TmMBP male3<br /> |
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| *MBP complex with saccharide | | *MBP complex with saccharide |
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| **[[1elj]] – MBP + glucose – ''Pyrococcus furiosus''<br /> | | **[[1elj]] – MBP + glucose – ''Pyrococcus furiosus''<br /> |
| **[[3jyr]] – MBP + α-acarbose – ''Salmonella enterica''<br /> | | **[[3jyr]] – MBP + α-acarbose – ''Salmonella enterica''<br /> |
| **[[2xd3]] – SpMBP + maltopentaose | | **[[2xd3]] – SpMBP + maltopentaose<br /> |
| | **[[6dtu]] - TmMBP male1 + tetraose <br /> |
| | **[[6dts]] - TmMBP male2 + maltotetraose <br /> |
| | **[[6dtq]] - TmMBP male3 + maltose <br /> |
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| *MBP complex with protein | | *MBP complex with protein |
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| **[[4nuf]] - MBP /nuclear receptor + orphan nuclear receptor peptide<br /> | | **[[4nuf]] - MBP /nuclear receptor + orphan nuclear receptor peptide<br /> |
| **[[5ldf]] - MBP + glutamine synthetase<br /> | | **[[5ldf]] - MBP + glutamine synthetase<br /> |
| | **[[6cxs]] - MBP + β-glucuronidase<br /> |
| **[[5jtr]], [[5jtq]] - MBP + SecB<br /> | | **[[5jtr]], [[5jtq]] - MBP + SecB<br /> |
| **[[5gs2]] - MBP + anti-MBP + anti-repebody + repebody<br /> | | **[[5gs2]] - MBP + anti-MBP + anti-repebody + repebody<br /> |
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| **[[5lem]], [[5lel]] - MBP + GFP + darpin<br /> | | **[[5lem]], [[5lel]] - MBP + GFP + darpin<br /> |
| **[[5e24]] - MBP + protein hairless + suppressor of hairless protein + DNA<br /> | | **[[5e24]] - MBP + protein hairless + suppressor of hairless protein + DNA<br /> |
| | **[[6dks]] - MBP + suppressor of hairless protein + DNA<br /> |
| **[[5cl1]] - MBP + frizzled 4<br /> | | **[[5cl1]] - MBP + frizzled 4<br /> |
| **[[5cbn]] - MBP + MBP3-16<br /> | | **[[5cbn]] - MBP + MBP3-16<br /> |
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Function
Maltose-binding protein (MBP) or ABC maltose transporter is part of the maltose/maltodextrin system in E. coli which is responsible of the catabolism of maltodextrin. MBP binds maltodextrin when the latter diffuses into the periplasm and passes it to proteins involved in active transport[1].
Relevance
MBP is widely used in molecular biology as it adds to the solubility of the proteins expressed in E. coli by being fused to them. The MBP-fusion protein can be purified by binding it to an amylose column followed by elution with maltose[2].
Structural highlights
The MBP sugar binding cleft is rich with protein-ligand hydrogen bonds[3]. Water molecules are shown as red spheres.
3D structures of maltose-binding protein
Maltose-binding protein 3D structures
- ↑ Kapust RB, Waugh DS. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 1999 Aug;8(8):1668-74. PMID:10452611 doi:https://dx.doi.org/10.1110/ps.8.8.1668
- ↑ Sun P, Tropea JE, Waugh DS. Enhancing the solubility of recombinant proteins in Escherichia coli by using hexahistidine-tagged maltose-binding protein as a fusion partner. Methods Mol Biol. 2011;705:259-74. doi: 10.1007/978-1-61737-967-3_16. PMID:21125392 doi:https://dx.doi.org/10.1007/978-1-61737-967-3_16
- ↑ Quiocho FA, Spurlino JC, Rodseth LE. Extensive features of tight oligosaccharide binding revealed in high-resolution structures of the maltodextrin transport/chemosensory receptor. Structure. 1997 Aug 15;5(8):997-1015. PMID:9309217
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3D structures of maltose-binding protein
Updated on 28-October-2019
{"openlevels":0}
- MBP
- Maltose-binding protein 3D structures, 1jw4, 1lls, 1omp - MBP
- 3sey, 3sex, 3sew, 3sev, 3seu, 3set, 3ses, 3ser, 2v93, 1zkb, 1n3x, 1peb , 1mpc, 3kjt, 1mdp, 1mdq - MBP (mutant)
- 2klf, 2d21 – MBP – NMR
- 1ziu – MBP (mutant) + Ni
- 1zjl – MBP (mutant) + Zn
- 1zmg - MBP (mutant) + Cu
- 4log - MBP /nuclear receptor
- 4ogm - MBP /pilin
- 4pe2 - MBP /pilA1
- 4khz - MBP MalK, MalF, MalG, MBP
- 3fh6 - MBP MalK, MalF, MalG
- 2xd2 – SpMBP – Streptococcus pneumoniae
- 6dtt - TmMBP male2 – Thermotoga maritima
- 6dtr - TmMBP male3
- MBP complex with saccharide
- 1n3w, 1nl5, 1mpd, 1iud, 1jvx, 1jvy - MBP (mutant) + maltose
- 1anf, 1jw5, 2xz3 - MBP + maltose
- 3mbp - MBP + maltotriose
- 4mbp - MBP + maltotetrose
- 1ez9 - MBP + maltodextrin
- 3hpi – MBP (mutant) + sucrose
- 1lax - MBP (mutant) + glucose
- 2h25, 1ezo, 1dmb - MBP + β-cyclodextrin
- 1ezp - MBP (mutant) + β-cyclodextrin
- 1fqa, 1fqb, 1fqc, 1fqd - MBP + maltotetraitol
- 3puy, 3puz - MBP MalK, MalF, MalG, MBP + maltose + AMPPNP
- 3rlf - MBP MalK, MalF, MalG, MBP + maltose + AMPPNP
- 4jbw - MBP MalK, MalF, MalG + EIIAGLC
- 3pv0 - MBP MalK, MalF, MalG, MBP + maltose
- 2r6g, 3puv, 3puw, 3pux, 4ki0 - MBP MalK, MalF, MalG, MBP + maltose + nucleotide
- 3ob4 - MBP AraH2 (mutant) + maltotriose
- 1elj – MBP + glucose – Pyrococcus furiosus
- 3jyr – MBP + α-acarbose – Salmonella enterica
- 2xd3 – SpMBP + maltopentaose
- 6dtu - TmMBP male1 + tetraose
- 6dts - TmMBP male2 + maltotetraose
- 6dtq - TmMBP male3 + maltose
- MBP complex with protein
- 3pgf – MBP (mutant) + synthetic antigen binder
- 2r6g, 4khz, 4ki0 - MBP + maltose transporter complex
- 3puv, 3puw, 3pux – MBP + maltose transporter complex + ADP
- 3rlf - MBP + maltose transporter complex + Mg-AMPPNP
- 1svx – MBP + ankyrin repeat protein OFF7
- 1nmu - MBP + ribosomal protein L30
- 1t0k - MBP + ribosomal protein L30 + RNA
- 3rum – MBP + ristocetin
- 4nuf - MBP /nuclear receptor + orphan nuclear receptor peptide
- 5ldf - MBP + glutamine synthetase
- 6cxs - MBP + β-glucuronidase
- 5jtr, 5jtq - MBP + SecB
- 5gs2 - MBP + anti-MBP + anti-repebody + repebody
- 5gru - MBP + diabody protein
- 5m15, 5m14, 5m13 - MBP + nanobody
- 5bk2, 5bk1, 5bjz - MBP + antibody
- 5fio, 5aq9 - MBP + darpin
- 5lem, 5lel - MBP + GFP + darpin
- 5e24 - MBP + protein hairless + suppressor of hairless protein + DNA
- 6dks - MBP + suppressor of hairless protein + DNA
- 5cl1 - MBP + frizzled 4
- 5cbn - MBP + MBP3-16
- 4wjv - MBP + ribosome assembly protein + ribosome biogenesis protein
References
