Vinculin

spinqualitylabelsall models Chicken full-length metavinculin, 1st6 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Vinculins (VCLs) are involved in adhesion by linking integrin molecules to the actin cytoskeleton. Its head domain (Vd1) can bind to talin or to alpha-actinin at their respective VCL Binding Sites (VBS). The protein raver1 RNA Recognition Motif (RRM) forms a complex with VCL or m-VCL. Metavinculin (m-VCL) is a splice version of VCL containing an extra ca. 70 amino acids in the C-terminal domain. Relevance Loss of VCL could be used as a prognostic factor for colorectal cancer se it promotes metastasis. Disease Mutation in m-VCL can yield cardiomyopathic phenotype. Structural highlights Vinculin Autoinhibition is achieved through a high affinity intramolecular interaction between tail (orange) and head (aqua) domains (1st6). Energetically, I997 is key to maintaining this autoinhibition.

3D Structures of Vinculin

Updated on 25-March-2020

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• Vinculin; Domains - head 140-327; tail 878-1066
  • talin, alpha-actinin – hVCL - human
    
  • 1st6 – hVCL head domain + raver1 RRM
    
  • 2ibf, 2hsq, 2gww - hVCL head domain + SfVCL binding sites from Shigella flexneri
    
  • 1ydi - hVCL head domain +hActinin VBS
    
  • 1syq, 1rkc, 1rke – hVCL head domain +hTalin VBS
    
  • 1qkr – hVCL tail domain
    
  • 3h2v - hVCL tail domain + raver1 RRM
    
  • 4pr9 - hVCL tail domain + lipid
    
  • 3jbi - hVCL tail domain + actin – Cryo-EM
    
  • 6nr7 - cVCL – chicken
    
  • 1t01 - cVCL head domain +mTalin VBS
    
  • 3zdl – cVCL head domain + amyloid β precursor protein N terminal
    
• Metavinculin; Domains - head 1-256; tail 959-1134
  • 6fuy - hm-VCL
    
  • 3rf3 - hm-VCL head domain + invasin IPAA
    
  • 3s90 - hm-VCL head domain + mTalin-1 peptide
    
  • 4dj9 - hm-VCL head domain + hTalin-1 peptide
    
  • 6fq4 - hm-VCL head domain + TARP peptide
    
  • 3tj5 - hm-VCL head domain + Sca-family protein peptide
    
  • 3tj6 - hm-VCL head domain + protein Ps 120 peptide
    
  • 4ehp, 5y04 - hm-VCL head domain + catenin α-1 residues 277-382
    
  • 3myi – hm-VCL tail domain
    
  • 5l0f, 5l0i, 5l0j - hm-VCL tail domain (mutant)
    
  • 5l0c, 5l0d - hm-VCL tail domain + lipid
    
  • 5l0g, 5l0h - hm-VCL tail domain (mutant) + lipid
    
  • 3jbk – hm-VCL tail domain + actin – Cryo-EM
    
  • 3vf0 – hm-VCL tail domain + ribonucleoprotein PTB-binding
    
  • 1st6 – cm-VCL
    
  • 2gdc – cm-VCL head domain +SfInvasin C-terminal
    
  • 1xwj - cm-VCL head domain +cTalin VBS3
    
  • 1zvz, 1zw2, 1zw3, 1u6h - cm-VCL head domain +cTalin rod
    
  • 6fq4 – cm-VCL head domain + TARP-VBS1
    
  • 4e17, 4e18 - cm-VCL head domain + catenin α-1 VCL-binding domain

References

• Created with the participation of Susan Craig.

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Alexander Berchansky, Jaime Prilusky, Michal Harel