5ebe

Structure of human sphingomyelinase phosphodiesterase like 3A (SMPDL3A) with 5' CMP

spinqualitylabelsall models 5ebe, resolution 3.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5ebe is a 3 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: C5P, GOL, MLI, NAG, RP5, SCN, ZN Related: 5ebb Gene: SMPDL3A, ASML3A (HUMAN) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Human sphingomyelinase phosphodiesterase like 3a (SMPDL3a) is a secreted enzyme that shares a conserved catalytic domain with human acid sphingomyelinase (aSMase), the enzyme carrying mutations causative of Niemann Pick disease. We have solved the structure of SMPDL3a revealing a calcineurin like fold. A dimetal site, glycosylation pattern and a disulphide bond network are likely to be conserved also in human aSMase. We show that the binuclear site of SMPDL3a is occupied by two Zn2+ ions and that excess of Zn2+ leads to inhibition of enzyme activity through binding to additional sites. As an extension of recent biochemical work we uncovered that SMPDL3a catalyses the hydrolysis of several modified nucleotides that include CDP-choline, CDP-ethanolamine and ADP-ribose, but not the aSMase substrate, sphingomyelin. We subsequently determined the structure of SMPDL3a in complex with the product 5'-cytidine monophosphate (CMP), a structure that is consistent with several distinct coordination modes of the substrate/product in the active site during the reaction cycle. Based on the structure of CMP complexes, we propose a phosphoryl transfer mechanism for SMPDL3a. Finally, a homology model of human aSMase was constructed to allow for the mapping of selected Niemann Pick disease mutations on a three-dimensional framework to guide further characterisation of their effects on aSMase function. This article is protected by copyright. All rights reserved. The structure and catalytic mechanism of Human Sphingomyelin Phosphodiesterase like 3a - an acid sphingomyelinase homolog with a novel nucleotide hydrolase activity.,Lim SM, Yeung K, Tresaugues L, Ling TH, Nordlund P FEBS J. 2016 Jan 18. doi: 10.1111/febs.13655. PMID:26783088 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Sphingomyelinase

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