Intersectin: Difference between revisions

Revision as of 08:09, 29 April 2020

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Human intersectin 1 4th SH3 domain (green, yellow, cyan, purple) complex with FCSHD2 SH3 2nd domain (grey, pink, light pink, orange) (PDB code 6gbu)

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Function

Intersectin (ITSN) is an endocytic multidomain scaffold protein in neurons and is important regulator of synaptic vescicles replenishment at hippocampal synapses. The replenishment is needed in order to sustain neurotransmission during periods of elevated activity. ITSN interacts with synapsin I through its SH3 domains^[1].

Relevance

ITSN 2 might be a potential biomarker for discrimination of severe acute pancreatitis to improve the prognosis of patients^[2]. Overexpression of ITSN 1 is implicated for human neurodegenerative diseases such as Down's syndrome and Alzheimer's disease^[3].

Structural highlights

The 3D structure of the complex between the SH3 domains of ITSN 1 and FCHSD2 shows extensive contact surface composed mainly of hydrophobic interactions^[4].

↑ . Correction for Gerth et al., Intersectin associates with synapsin and regulates its nanoscale localization and function. Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):E11060. doi:, 10.1073/pnas.1720226115. Epub 2017 Dec 11. PMID:29229830 doi:https://dx.doi.org/10.1073/pnas.1720226115
↑ Li J, Bu X, Chen X, Xiong P, Chen Z, Yu L. Predictive value of long non-coding RNA intersectin 1-2 for occurrence and in-hospital mortality of severe acute pancreatitis. J Clin Lab Anal. 2019 Dec 27:e23170. doi: 10.1002/jcla.23170. PMID:31880027 doi:https://dx.doi.org/10.1002/jcla.23170
↑ Hunter MP, Nelson M, Kurzer M, Wang X, Kryscio RJ, Head E, Pinna G, O'Bryan JP. Intersectin 1 contributes to phenotypes in vivo: implications for Down's syndrome. Neuroreport. 2011 Oct 26;22(15):767-72. doi: 10.1097/WNR.0b013e32834ae348. PMID:21876463 doi:https://dx.doi.org/10.1097/WNR.0b013e32834ae348
↑ Almeida-Souza L, Frank RAW, Garcia-Nafria J, Colussi A, Gunawardana N, Johnson CM, Yu M, Howard G, Andrews B, Vallis Y, McMahon HT. A Flat BAR Protein Promotes Actin Polymerization at the Base of Clathrin-Coated Pits. Cell. 2018 Jun 4. pii: S0092-8674(18)30597-X. doi: 10.1016/j.cell.2018.05.020. PMID:29887380 doi:https://dx.doi.org/10.1016/j.cell.2018.05.020

3D structures of intersectin

Updated on 29-April-2020 {{#tree:id=OrganizedByTopic|openlevels=0|

Intersectin 1

- 3fia - hITSN1 EH domain 1-111
- 2khn - hITSN1 EH domain - NMR
- 2kgr - hITSN1 residues 210-312 - NMR
- 6h5t - hITSN1 1st SH3 domain 741-835
- 4iim - hITSN1 2nd SH3 domain + peptide
- 6gbu - hITSN1 4th SH3 domain 1055-1133 + FCHSD2
- 1ki1 - hITSN1 DBL+PH domains 1229-1580 + Cdc42
- 3qbv - hITSN1 DBL+PH domains (mutant) + Cdc42
- 3jv3 - mITSN1 5th SH3+DH domains 1151-1431

Intersectin 2

- 1uff - hITSN2 1st SH3 domain 761-841 - NMR
- 1j3t - hITSN2 2nd SH3 domain 897-957 - NMR
- 4iio - hITSN2 2nd SH3 domain + peptide
- 1uhf - hITSN2 3rd SH3 domain 982-1037 - NMR
- 1ue9 - hITSN2 4th SH3 domain 1055-1121 - NMR
- 1udl - hITSN2 5th SH3 domain 1102-1186 - NMR
- 3gf9 - hITSN2 RhoGEF domain 1102-1378
- 3jzy - hITSN2 C2 domain 1173-1664

References

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] . Correction for Gerth et al., Intersectin associates with synapsin and regulates its nanoscale localization and function. Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):E11060. doi:, 10.1073/pnas.1720226115. Epub 2017 Dec 11. PMID:29229830 doi:https://dx.doi.org/10.1073/pnas.1720226115

[2] Li J, Bu X, Chen X, Xiong P, Chen Z, Yu L. Predictive value of long non-coding RNA intersectin 1-2 for occurrence and in-hospital mortality of severe acute pancreatitis. J Clin Lab Anal. 2019 Dec 27:e23170. doi: 10.1002/jcla.23170. PMID:31880027 doi:https://dx.doi.org/10.1002/jcla.23170

[3] Hunter MP, Nelson M, Kurzer M, Wang X, Kryscio RJ, Head E, Pinna G, O'Bryan JP. Intersectin 1 contributes to phenotypes in vivo: implications for Down's syndrome. Neuroreport. 2011 Oct 26;22(15):767-72. doi: 10.1097/WNR.0b013e32834ae348. PMID:21876463 doi:https://dx.doi.org/10.1097/WNR.0b013e32834ae348

[4] Almeida-Souza L, Frank RAW, Garcia-Nafria J, Colussi A, Gunawardana N, Johnson CM, Yu M, Howard G, Andrews B, Vallis Y, McMahon HT. A Flat BAR Protein Promotes Actin Polymerization at the Base of Clathrin-Coated Pits. Cell. 2018 Jun 4. pii: S0092-8674(18)30597-X. doi: 10.1016/j.cell.2018.05.020. PMID:29887380 doi:https://dx.doi.org/10.1016/j.cell.2018.05.020

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@@ Line 1: / Line 1: @@
-<StructureSection load='6gbu' size='340' side='right' caption='Caption for this structure' scene=''>
+<StructureSection load='6gbu' size='340' side='right' caption='Human intersectin 1 4th SH3 domain (green, yellow, cyan, purple) complex with FCSHD2 SH3 2nd domain (grey, pink, light pink, orange) (PDB code [[6gbu]])' scene=''>
 == Function ==
 '''Intersectin''' (ITSN) is an endocytic multidomain scaffold protein in neurons and is important regulator of synaptic vescicles replenishment at hippocampal synapses.  The replenishment is needed in order to sustain neurotransmission during periods of elevated activity. ITSN interacts with synapsin I through its SH3 domains<ref>PMID:29229830</ref>.
-== Disease ==
 == Relevance ==
@@ Line 13: / Line 11: @@
 == Structural highlights ==
+The 3D structure of the complex between the SH3 domains of ITSN 1 and FCHSD2 shows extensive contact surface composed mainly of hydrophobic interactions<ref>PMID:29887380</ref>.
 </StructureSection>

Intersectin: Difference between revisions

Revision as of 08:09, 29 April 2020

Function

Relevance

Structural highlights

3D structures of intersectin

References

Proteopedia Page Contributors and Editors (what is this?)

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