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| *Metavinculin; Domains - head 1-256; tail 959-1134 | | *Metavinculin; Domains - head 1-256; tail 959-1134 |
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| **[[6fuy]] - hm-VCL <br /> | | **[[6fuy]] - hVCL <br /> |
| **[[3rf3]] - hm-VCL head domain + invasin IPAA<br /> | | **[[3rf3]] - hVCL head domain + invasin IPAA<br /> |
| **[[3s90]] - hm-VCL head domain + mTalin-1 peptide<br /> | | **[[3s90]] - hVCL head domain + mTalin-1 peptide<br /> |
| **[[4dj9]] - hm-VCL head domain + hTalin-1 peptide <br /> | | **[[4dj9]] - hVCL head domain + hTalin-1 peptide <br /> |
| **[[6fq4]] - hm-VCL head domain + TARP peptide <br /> | | **[[6fq4]] - hVCL head domain + TARP peptide <br /> |
| **[[3tj5]] - hm-VCL head domain + Sca-family protein peptide<br /> | | **[[3tj5]] - hVCL head domain + Sca-family protein peptide<br /> |
| **[[3tj6]] - hm-VCL head domain + protein Ps 120 peptide<br /> | | **[[3tj6]] - hVCL head domain + protein Ps 120 peptide<br /> |
| **[[4ehp]], [[5y04]] - hm-VCL head domain + catenin α-1 residues 277-382<br /> | | **[[4ehp]], [[5y04]] - hVCL head domain + catenin α-1 residues 277-382<br /> |
| **[[3myi]] – hm-VCL tail domain <br /> | | **[[3myi]] – hVCL tail domain <br /> |
| **[[5l0f]], [[5l0i]], [[5l0j]] - hm-VCL tail domain (mutant)<br /> | | **[[5l0f]], [[5l0i]], [[5l0j]] - hVCL tail domain (mutant)<br /> |
| **[[5l0c]], [[5l0d]] - hm-VCL tail domain + lipid<br /> | | **[[5l0c]], [[5l0d]] - hVCL tail domain + lipid<br /> |
| **[[5l0g]], [[5l0h]] - hm-VCL tail domain (mutant) + lipid<br /> | | **[[5l0g]], [[5l0h]] - hVCL tail domain (mutant) + lipid<br /> |
| **[[3jbk]] – hm-VCL tail domain + actin – Cryo-EM<br /> | | **[[3jbk]] – hVCL tail domain + actin – Cryo-EM<br /> |
| **[[3vf0]] – hm-VCL tail domain + ribonucleoprotein PTB-binding<br /> | | **[[6upw]] - mVCL head domain + catenin α-1<br /> |
| **[[1st6]] – cm-VCL<br /> | | **[[3vf0]] – hVCL tail domain + ribonucleoprotein PTB-binding<br /> |
| **[[2gdc]] – cm-VCL head domain +SfInvasin C-terminal <br /> | | **[[1st6]] – cVCL<br /> |
| **[[1xwj]] - cm-VCL head domain +cTalin VBS3<br /> | | **[[2gdc]] – cVCL head domain +SfInvasin C-terminal <br /> |
| **[[1zvz]], [[1zw2]], [[1zw3]], [[1u6h]] - cm-VCL head domain +cTalin rod<br /> | | **[[1xwj]] - cVCL head domain +cTalin VBS3<br /> |
| **[[6fq4]] – cm-VCL head domain + TARP-VBS1<br /> | | **[[1zvz]], [[1zw2]], [[1zw3]], [[1u6h]] - cVCL head domain +cTalin rod<br /> |
| **[[4e17]], [[4e18]] - cm-VCL head domain + catenin α-1 VCL-binding domain | | **[[6fq4]] – cVCL head domain + TARP-VBS1<br /> |
| | **[[4e17]], [[4e18]] - cVCL head domain + catenin α-1 VCL-binding domain |
| }} | | }} |
| ==References== | | ==References== |
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Function
Vinculins (VCLs) are involved in adhesion by linking integrin molecules to the actin cytoskeleton. Its head domain (Vd1) can bind to talin or to alpha-actinin at their respective VCL Binding Sites (VBS)[1]. The protein raver1 RNA Recognition Motif (RRM) forms a complex with VCL or m-VCL. Metavinculin (m-VCL) is a splice version of VCL containing an extra ca. 70 amino acids in the C-terminal domain.
Relevance
Loss of VCL could be used as a prognostic factor for colorectal cancer se it promotes metastasis[2].
Disease
Mutation in m-VCL can yield cardiomyopathic phenotype[3].
Structural highlights
Vinculin Autoinhibition is achieved through a high affinity intramolecular interaction between tail (orange) and head (aqua) domains (1st6). Energetically, I997 is key to maintaining this autoinhibition.
- ↑ Palovuori R, Eskelinen S. Role of vinculin in the maintenance of cell-cell contacts in kidney epithelial MDBK cells. Eur J Cell Biol. 2000 Dec;79(12):961-74. PMID:11152287 doi:https://dx.doi.org/10.1078/0171-9335-00120
- ↑ Li T, Guo H, Song Y, Zhao X, Shi Y, Lu Y, Hu S, Nie Y, Fan D, Wu K. Loss of vinculin and membrane-bound beta-catenin promotes metastasis and predicts poor prognosis in colorectal cancer. Mol Cancer. 2014 Dec 11;13:263. doi: 10.1186/1476-4598-13-263. PMID:25496021 doi:https://dx.doi.org/10.1186/1476-4598-13-263
- ↑ Vasile VC, Will ML, Ommen SR, Edwards WD, Olson TM, Ackerman MJ. Identification of a metavinculin missense mutation, R975W, associated with both hypertrophic and dilated cardiomyopathy. Mol Genet Metab. 2006 Feb;87(2):169-74. Epub 2005 Oct 19. PMID:16236538 doi:S1096-7192(05)00258-1
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3D Structures of Vinculin
Updated on 11-January-2021
{"openlevels":0}
- Vinculin; Domains - head 140-327; tail 878-1066
- talin, alpha-actinin – hVCL - human
- 1st6 – hVCL head domain + raver1 RRM
- 2ibf, 2hsq, 2gww - hVCL head domain + SfVCL binding sites from Shigella flexneri
- 1ydi - hVCL head domain +hActinin VBS
- 1syq, 1rkc, 1rke – hVCL head domain +hTalin VBS
- 1qkr – hVCL tail domain
- 3h2v - hVCL tail domain + raver1 RRM
- 4pr9 - hVCL tail domain + lipid
- 3jbi - hVCL tail domain + actin – Cryo-EM
- 6nr7 - cVCL – chicken
- 1t01 - cVCL head domain +mTalin VBS
- 3zdl – cVCL head domain + amyloid β precursor protein N terminal
- Metavinculin; Domains - head 1-256; tail 959-1134
- 6fuy - hVCL
- 3rf3 - hVCL head domain + invasin IPAA
- 3s90 - hVCL head domain + mTalin-1 peptide
- 4dj9 - hVCL head domain + hTalin-1 peptide
- 6fq4 - hVCL head domain + TARP peptide
- 3tj5 - hVCL head domain + Sca-family protein peptide
- 3tj6 - hVCL head domain + protein Ps 120 peptide
- 4ehp, 5y04 - hVCL head domain + catenin α-1 residues 277-382
- 3myi – hVCL tail domain
- 5l0f, 5l0i, 5l0j - hVCL tail domain (mutant)
- 5l0c, 5l0d - hVCL tail domain + lipid
- 5l0g, 5l0h - hVCL tail domain (mutant) + lipid
- 3jbk – hVCL tail domain + actin – Cryo-EM
- 6upw - mVCL head domain + catenin α-1
- 3vf0 – hVCL tail domain + ribonucleoprotein PTB-binding
- 1st6 – cVCL
- 2gdc – cVCL head domain +SfInvasin C-terminal
- 1xwj - cVCL head domain +cTalin VBS3
- 1zvz, 1zw2, 1zw3, 1u6h - cVCL head domain +cTalin rod
- 6fq4 – cVCL head domain + TARP-VBS1
- 4e17, 4e18 - cVCL head domain + catenin α-1 VCL-binding domain
References
