3o3r: Difference between revisions

Revision as of 05:27, 10 August 2022

Crystal Structure of AKR1B14 in complex with NADP

Structural highlights

3o3r is a 2 chain structure with sequence from Buffalo rat. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	NAP
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ALD1_RAT] Reduces a broad range of aliphatic and aromatic aldehydes to the corresponding alcohols. May play a role in the metabolism of xenobiotic aromatic aldehydes.^[1]

Publication Abstract from PubMed

Rat aldose reductase-like protein (AKR1B14) is the ortholog of mouse vas deferens protein (AKR1B7) playing roles in detoxification of reactive aldehydes and synthesis of prostaglandin F(2alpha). The crystal structure of the binary complex (AKR1B14-NADPH) was determined at 1.86A resolution, and showed that the adenine ring and the 2'-phosphate group of the coenzyme formed pi-stacking and electrostatic interactions with the imidazole ring and ND1 atom, respectively, of His269, which is not conserved in other aldose reductase-like proteins. The interactions were supported by site-directed mutagenesis of His269 to Arg, Phe and Met, which increased the K(m) for NADPH by 4, 7 and 127-fold, respectively. This is the first report of the tertiary structure of a rodent AKR1B7 ortholog, which describes the role of a novel dual interaction for the non-conserved His269 in coenzyme binding.

Structure of rat aldose reductase-like protein AKR1B14 holoenzyme: Probing the role of His269 in coenzyme binding by site-directed mutagenesis.,Sundaram K, Dhagat U, Endo S, Chung R, Matsunaga T, Hara A, El-Kabbani O Bioorg Med Chem Lett. 2011 Jan 15;21(2):801-4. Epub 2010 Nov 24. PMID:21168333^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Endo S, Matsunaga T, Fujita A, Tajima K, El-Kabbani O, Hara A. Rat aldose reductase-like protein (AKR1B14) efficiently reduces the lipid peroxidation product 4-oxo-2-nonenal. Biol Pharm Bull. 2010;33(11):1886-90. PMID:21048316
↑ Sundaram K, Dhagat U, Endo S, Chung R, Matsunaga T, Hara A, El-Kabbani O. Structure of rat aldose reductase-like protein AKR1B14 holoenzyme: Probing the role of His269 in coenzyme binding by site-directed mutagenesis. Bioorg Med Chem Lett. 2011 Jan 15;21(2):801-4. Epub 2010 Nov 24. PMID:21168333 doi:10.1016/j.bmcl.2010.11.086

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Endo S, Matsunaga T, Fujita A, Tajima K, El-Kabbani O, Hara A. Rat aldose reductase-like protein (AKR1B14) efficiently reduces the lipid peroxidation product 4-oxo-2-nonenal. Biol Pharm Bull. 2010;33(11):1886-90. PMID:21048316

[2] Sundaram K, Dhagat U, Endo S, Chung R, Matsunaga T, Hara A, El-Kabbani O. Structure of rat aldose reductase-like protein AKR1B14 holoenzyme: Probing the role of His269 in coenzyme binding by site-directed mutagenesis. Bioorg Med Chem Lett. 2011 Jan 15;21(2):801-4. Epub 2010 Nov 24. PMID:21168333 doi:10.1016/j.bmcl.2010.11.086

[1]

[2]

@@ Line 3: / Line 3: @@
 <StructureSection load='3o3r' size='340' side='right'caption='[[3o3r]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3o3r]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3R OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=3O3R FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3o3r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3O3R FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=3o3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3r OCA], [http://pdbe.org/3o3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3o3r RCSB], [http://www.ebi.ac.uk/pdbsum/3o3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3o3r ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3o3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o3r OCA], [https://pdbe.org/3o3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3o3r RCSB], [https://www.ebi.ac.uk/pdbsum/3o3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3o3r ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ALD1_RAT ALD1_RAT]] Reduces a broad range of aliphatic and aromatic aldehydes to the corresponding alcohols. May play a role in the metabolism of xenobiotic aromatic aldehydes.<ref>PMID:21048316</ref>
+[[https://www.uniprot.org/uniprot/ALD1_RAT ALD1_RAT]] Reduces a broad range of aliphatic and aromatic aldehydes to the corresponding alcohols. May play a role in the metabolism of xenobiotic aromatic aldehydes.<ref>PMID:21048316</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==

3o3r: Difference between revisions

Revision as of 05:27, 10 August 2022

Crystal Structure of AKR1B14 in complex with NADP

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools