4ady: Difference between revisions

Revision as of 05:31, 25 August 2022

Crystal structure of 26S proteasome subunit Rpn2

Structural highlights

4ady is a 2 chain structure with sequence from Atcc 18824. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:	MSE
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RPN2_YEAST] Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.^[1]

Publication Abstract from PubMed

The 26S proteasome proteolyses ubiquitylated proteins and is assembled from a 20S proteolytic core and two 19S regulatory particles (19S-RP). The 19S-RP scaffolding subunits Rpn1 and Rpn2 function to engage ubiquitin receptors. Rpn1 and Rpn2 are characterized by eleven tandem copies of a 35-40 amino acid repeat motif termed the proteasome/cyclosome (PC) repeat. Here, we reveal that the eleven PC repeats of Rpn2 form a closed toroidal structure incorporating two concentric rings of alpha helices encircling two axial alpha helices. A rod-like N-terminal domain consisting of 17 stacked alpha helices and a globular C-terminal domain emerge from one face of the toroid. Rpn13, an ubiquitin receptor, binds to the C-terminal 20 residues of Rpn2. Rpn1 adopts a similar conformation to Rpn2 but differs in the orientation of its rod-like N-terminal domain. These findings have implications for understanding how 19S-RPs recognize, unfold, and deliver ubiquitylated substrates to the 20S core.

The Structure of the 26S Proteasome Subunit Rpn2 Reveals Its PC Repeat Domain as a Closed Toroid of Two Concentric alpha-Helical Rings.,He J, Kulkarni K, da Fonseca PC, Krutauz D, Glickman MH, Barford D, Morris EP Structure. 2012 Mar 7;20(3):513-21. PMID:22405010^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Glickman MH, Rubin DM, Fried VA, Finley D. The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol. 1998 Jun;18(6):3149-62. PMID:9584156
↑ He J, Kulkarni K, da Fonseca PC, Krutauz D, Glickman MH, Barford D, Morris EP. The Structure of the 26S Proteasome Subunit Rpn2 Reveals Its PC Repeat Domain as a Closed Toroid of Two Concentric alpha-Helical Rings. Structure. 2012 Mar 7;20(3):513-21. PMID:22405010 doi:10.1016/j.str.2011.12.015

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OCA

[1] Glickman MH, Rubin DM, Fried VA, Finley D. The regulatory particle of the Saccharomyces cerevisiae proteasome. Mol Cell Biol. 1998 Jun;18(6):3149-62. PMID:9584156

[2] He J, Kulkarni K, da Fonseca PC, Krutauz D, Glickman MH, Barford D, Morris EP. The Structure of the 26S Proteasome Subunit Rpn2 Reveals Its PC Repeat Domain as a Closed Toroid of Two Concentric alpha-Helical Rings. Structure. 2012 Mar 7;20(3):513-21. PMID:22405010 doi:10.1016/j.str.2011.12.015

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@@ Line 3: / Line 3: @@
 <StructureSection load='4ady' size='340' side='right'caption='[[4ady]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ady]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ADY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ADY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ady]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ADY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ADY FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ady FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ady OCA], [http://pdbe.org/4ady PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ady RCSB], [http://www.ebi.ac.uk/pdbsum/4ady PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ady ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ady FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ady OCA], [https://pdbe.org/4ady PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ady RCSB], [https://www.ebi.ac.uk/pdbsum/4ady PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ady ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/RPN2_YEAST RPN2_YEAST]] Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:9584156</ref>
+[[https://www.uniprot.org/uniprot/RPN2_YEAST RPN2_YEAST]] Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.<ref>PMID:9584156</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 20: @@
 ==See Also==
-*[[Proteasome|Proteasome]]
+*[[Proteasome 3D structures|Proteasome 3D structures]]
 == References ==
 <references/>

4ady: Difference between revisions

Revision as of 05:31, 25 August 2022

Crystal structure of 26S proteasome subunit Rpn2

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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