4dfu: Difference between revisions

Revision as of 08:16, 21 September 2022

Inhibition of an antibiotic resistance enzyme: crystal structure of aminoglycoside phosphotransferase APH(2")-ID/APH(2")-IVA in complex with kanamycin inhibited with quercetin

Structural highlights

4dfu is a 2 chain structure with sequence from Enterococcus casseliflavus. This structure supersedes the now removed PDB entries 3vcq and 3r82. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	CL, KAN, MSE, QUE
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[O68183_ENTCA]

Publication Abstract from PubMed

Kinase-mediated resistance to antibiotics is a significant clinical challenge. These enzymes share a common protein fold characteristic of Ser/Thr/Tyr protein kinases. We screened 14 antibiotic resistance kinases against 80 chemically diverse protein kinase inhibitors to map resistance kinase chemical space. The screens identified molecules with both broad and narrow inhibition profiles, proving that protein kinase inhibitors offer privileged chemical matter with the potential to block antibiotic resistance. One example is the flavonol quercetin, which inhibited a number of resistance kinases in vitro and in vivo. This activity was rationalized by determination of the crystal structure of the aminoglycoside kinase APH(2)-IVa in complex with quercetin and its antibiotic substrate kanamycin. Our data demonstrate that protein kinase inhibitors offer chemical scaffolds that can block antibiotic resistance, providing leads for co-drug design.

A small molecule discrimination map of the antibiotic resistance kinome.,Shakya T, Stogios PJ, Waglechner N, Evdokimova E, Ejim L, Blanchard JE, McArthur AG, Savchenko A, Wright GD Chem Biol. 2011 Dec 23;18(12):1591-601. PMID:22195561^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shakya T, Stogios PJ, Waglechner N, Evdokimova E, Ejim L, Blanchard JE, McArthur AG, Savchenko A, Wright GD. A small molecule discrimination map of the antibiotic resistance kinome. Chem Biol. 2011 Dec 23;18(12):1591-601. PMID:22195561 doi:10.1016/j.chembiol.2011.10.018

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OCA

[1] Shakya T, Stogios PJ, Waglechner N, Evdokimova E, Ejim L, Blanchard JE, McArthur AG, Savchenko A, Wright GD. A small molecule discrimination map of the antibiotic resistance kinome. Chem Biol. 2011 Dec 23;18(12):1591-601. PMID:22195561 doi:10.1016/j.chembiol.2011.10.018

[1]

@@ Line 1: / Line 1: @@
 ==Inhibition of an antibiotic resistance enzyme: crystal structure of aminoglycoside phosphotransferase APH(2")-ID/APH(2")-IVA in complex with kanamycin inhibited with quercetin==
-<StructureSection load='4dfu' size='340' side='right' caption='[[4dfu]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
+<StructureSection load='4dfu' size='340' side='right'caption='[[4dfu]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4dfu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptococcus_casseliflavus"_vaughan_et_al._1979 "streptococcus casseliflavus" vaughan et al. 1979]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3vcq 3vcq] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3r82 3r82]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DFU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DFU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4dfu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_casseliflavus Enterococcus casseliflavus]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3vcq 3vcq] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3r82 3r82]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DFU FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=KAN:KANAMYCIN+A'>KAN</scene>, <scene name='pdbligand=QUE:3,5,7,3,4-PENTAHYDROXYFLAVONE'>QUE</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=KAN:KANAMYCIN+A'>KAN</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=QUE:3,5,7,3,4-PENTAHYDROXYFLAVONE'>QUE</scene></td></tr>
-<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4dfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dfu OCA], [https://pdbe.org/4dfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4dfu RCSB], [https://www.ebi.ac.uk/pdbsum/4dfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4dfu ProSAT]</span></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4dbx|4dbx]], [[4de4|4de4]], [[4dfb|4dfb]]</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">APH(2")-Id ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=37734 "Streptococcus casseliflavus" Vaughan et al. 1979])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4dfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4dfu OCA], [http://pdbe.org/4dfu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4dfu RCSB], [http://www.ebi.ac.uk/pdbsum/4dfu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4dfu ProSAT]</span></td></tr>
 </table>
+== Function ==
+[[https://www.uniprot.org/uniprot/O68183_ENTCA O68183_ENTCA]]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 20: @@
 ==See Also==
-*[[Phosphotransferase|Phosphotransferase]]
+*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Streptococcus casseliflavus vaughan et al. 1979]]
+[[Category: Enterococcus casseliflavus]]
-[[Category: Anderson, W F]]
+[[Category: Large Structures]]
-[[Category: Structural genomic]]
+[[Category: Anderson WF]]
-[[Category: Dong, A]]
+[[Category: Di Leo R]]
-[[Category: Egorova, E]]
+[[Category: Dong A]]
-[[Category: Evdokimova, E]]
+[[Category: Egorova E]]
-[[Category: Leo, R Di]]
+[[Category: Evdokimova E]]
-[[Category: Li, H]]
+[[Category: Li H]]
-[[Category: Minasov, G]]
+[[Category: Minasov G]]
-[[Category: Savchenko, A]]
+[[Category: Savchenko A]]
-[[Category: Shakya, T]]
+[[Category: Shakya T]]
-[[Category: Stogios, P J]]
+[[Category: Stogios PJ]]
-[[Category: Wright, G D]]
+[[Category: Wright GD]]
-[[Category: Aminoglycoside phosphotransferase]]
-[[Category: Aminoglycoside]]
-[[Category: Antibotic]]
-[[Category: Csgid]]
-[[Category: Eukaryotic protein kinase-like fold]]
-[[Category: Flavanoid]]
-[[Category: Intracellular]]
-[[Category: Kanamycin]]
-[[Category: Kinase]]
-[[Category: Quercetin]]
-[[Category: Transferase]]
-[[Category: Transferase-antibiotic-inhibitor complex]]
-[[Category: Transferase-antibotic-inhibitor complex]]

4dfu: Difference between revisions

Revision as of 08:16, 21 September 2022

Inhibition of an antibiotic resistance enzyme: crystal structure of aminoglycoside phosphotransferase APH(2")-ID/APH(2")-IVA in complex with kanamycin inhibited with quercetin

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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