4o26: Difference between revisions

Revision as of 08:53, 18 January 2023

Crystal structure of the TRBD domain of TERT and the CR4/5 of TR

Structural highlights

4o26 is a 4 chain structure with sequence from Oryzias latipes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TERT_ORYLA Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.^[1] ^[2]

Publication Abstract from PubMed

Telomerase is a large ribonucleoprotein complex minimally composed of a catalytic telomerase reverse transcriptase (TERT) and an RNA component (TR) that provides the template for telomeric DNA synthesis. However, it remains unclear how TERT and TR assemble into a functional telomerase. Here we report the crystal structure of the conserved regions 4 and 5 (CR4/5) of TR in complex with the TR-binding domain (TRBD) of TERT from the teleost fish Oryzias latipes. The structure shows that CR4/5 adopts an L-shaped three-way-junction conformation with its two arms clamping onto TRBD. Both the sequence and conformation of CR4/5 are required for the interaction. Our structural and mutational analyses suggest that the observed CR4/5-TRBD recognition is common to most eukaryotes, and CR4/5 in vertebrate TR might have a similar role in telomerase regulation as that of stem-loop IV in Tetrahymena TR.

Structural basis for protein-RNA recognition in telomerase.,Huang J, Brown AF, Wu J, Xue J, Bley CJ, Rand DP, Wu L, Zhang R, Chen JJ, Lei M Nat Struct Mol Biol. 2014 Jun;21(6):507-12. doi: 10.1038/nsmb.2819. Epub 2014 May, 4. PMID:24793650^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xie M, Mosig A, Qi X, Li Y, Stadler PF, Chen JJ. Structure and function of the smallest vertebrate telomerase RNA from teleost fish. J Biol Chem. 2008 Jan 25;283(4):2049-59. doi: 10.1074/jbc.M708032200. Epub 2007 , Nov 26. PMID:18039659 doi:https://dx.doi.org/10.1074/jbc.M708032200
↑ Bley CJ, Qi X, Rand DP, Borges CR, Nelson RW, Chen JJ. RNA-protein binding interface in the telomerase ribonucleoprotein. Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20333-8. doi: , 10.1073/pnas.1100270108. Epub 2011 Nov 28. PMID:22123986 doi:https://dx.doi.org/10.1073/pnas.1100270108
↑ Huang J, Brown AF, Wu J, Xue J, Bley CJ, Rand DP, Wu L, Zhang R, Chen JJ, Lei M. Structural basis for protein-RNA recognition in telomerase. Nat Struct Mol Biol. 2014 Jun;21(6):507-12. doi: 10.1038/nsmb.2819. Epub 2014 May, 4. PMID:24793650 doi:https://dx.doi.org/10.1038/nsmb.2819

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OCA

[1] Xie M, Mosig A, Qi X, Li Y, Stadler PF, Chen JJ. Structure and function of the smallest vertebrate telomerase RNA from teleost fish. J Biol Chem. 2008 Jan 25;283(4):2049-59. doi: 10.1074/jbc.M708032200. Epub 2007 , Nov 26. PMID:18039659 doi:https://dx.doi.org/10.1074/jbc.M708032200

[2] Bley CJ, Qi X, Rand DP, Borges CR, Nelson RW, Chen JJ. RNA-protein binding interface in the telomerase ribonucleoprotein. Proc Natl Acad Sci U S A. 2011 Dec 20;108(51):20333-8. doi: , 10.1073/pnas.1100270108. Epub 2011 Nov 28. PMID:22123986 doi:https://dx.doi.org/10.1073/pnas.1100270108

[3] Huang J, Brown AF, Wu J, Xue J, Bley CJ, Rand DP, Wu L, Zhang R, Chen JJ, Lei M. Structural basis for protein-RNA recognition in telomerase. Nat Struct Mol Biol. 2014 Jun;21(6):507-12. doi: 10.1038/nsmb.2819. Epub 2014 May, 4. PMID:24793650 doi:https://dx.doi.org/10.1038/nsmb.2819

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[2]

[3]

@@ Line 3: / Line 3: @@
 <StructureSection load='4o26' size='340' side='right'caption='[[4o26]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4o26]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_killifish Japanese killifish]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O26 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O26 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4o26]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryzias_latipes Oryzias latipes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O26 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O26 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TERT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=8090 Japanese killifish])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o26 OCA], [https://pdbe.org/4o26 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o26 RCSB], [https://www.ebi.ac.uk/pdbsum/4o26 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o26 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o26 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o26 OCA], [http://pdbe.org/4o26 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o26 RCSB], [http://www.ebi.ac.uk/pdbsum/4o26 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o26 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/TERT_ORYLA TERT_ORYLA] Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. It elongates telomeres. It is a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme.<ref>PMID:18039659</ref> <ref>PMID:22123986</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 20: @@
 ==See Also==
-*[[Telomerase|Telomerase]]
+*[[Telomerase 3D structures|Telomerase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Japanese killifish]]
 [[Category: Large Structures]]
-[[Category: Huang, J]]
+[[Category: Oryzias latipes]]
-[[Category: Lei, M]]
+[[Category: Huang J]]
-[[Category: Wu, J]]
+[[Category: Lei M]]
-[[Category: Protein-rna interaction]]
+[[Category: Wu J]]
-[[Category: Rna binding protein-rna complex]]
-[[Category: Telomerase]]
-[[Category: Telomerase rna]]

4o26: Difference between revisions

Revision as of 08:53, 18 January 2023

Crystal structure of the TRBD domain of TERT and the CR4/5 of TR

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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