4ru5: Difference between revisions

Revision as of 08:28, 8 March 2023

Crystal Structure of the Pseudomonas phage phi297 tailspike gp61

Structural highlights

4ru5 is a 3 chain structure with sequence from Pseudomonas phage phi297. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	ACT, CA, EDO, NA
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

H2BD96_9CAUD

Publication Abstract from PubMed

Pseudomonas phage LKA1 of the subfamily Autographivirinae encodes a tailspike protein (LKA1gp49) which binds and cleaves B-band LPS (O-specific antigen, OSA) of Pseudomonas aeruginosa PAO1. The crystal structure of LKA1gp49 catalytic domain consists of a beta-helix, an insertion domain and a C-terminal discoidin-like domain. The putative substrate binding and processing site is located on the face of the beta-helix whereas the C-terminal domain is likely involved in carbohydrates binding. NMR spectroscopy and mass spectrometry analyses of degraded LPS (OSA) fragments show an O5 serotype-specific polysaccharide lyase specificity. LKA1gp49 reduces virulence in an in vivo Galleria mellonella infection model and sensitizes P. aeruginosa to serum complement activity. This enzyme causes biofilm degradation and does not affect the activity of ciprofloxacin and gentamicin. This is the first comprehensive report on LPS-degrading lyase derived from a Pseudomonas phage. Biological properties reveal a potential towards its applications in antimicrobial design and as a microbiological or biotechnological tool.

The O-specific polysaccharide lyase from the phage LKA1 tailspike reduces Pseudomonas virulence.,Olszak T, Shneider MM, Latka A, Maciejewska B, Browning C, Sycheva LV, Cornelissen A, Danis-Wlodarczyk K, Senchenkova SN, Shashkov AS, Gula G, Arabski M, Wasik S, Miroshnikov KA, Lavigne R, Leiman PG, Knirel YA, Drulis-Kawa Z Sci Rep. 2017 Nov 24;7(1):16302. doi: 10.1038/s41598-017-16411-4. PMID:29176754^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Olszak T, Shneider MM, Latka A, Maciejewska B, Browning C, Sycheva LV, Cornelissen A, Danis-Wlodarczyk K, Senchenkova SN, Shashkov AS, Gula G, Arabski M, Wasik S, Miroshnikov KA, Lavigne R, Leiman PG, Knirel YA, Drulis-Kawa Z. The O-specific polysaccharide lyase from the phage LKA1 tailspike reduces Pseudomonas virulence. Sci Rep. 2017 Nov 24;7(1):16302. doi: 10.1038/s41598-017-16411-4. PMID:29176754 doi:https://dx.doi.org/10.1038/s41598-017-16411-4

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Olszak T, Shneider MM, Latka A, Maciejewska B, Browning C, Sycheva LV, Cornelissen A, Danis-Wlodarczyk K, Senchenkova SN, Shashkov AS, Gula G, Arabski M, Wasik S, Miroshnikov KA, Lavigne R, Leiman PG, Knirel YA, Drulis-Kawa Z. The O-specific polysaccharide lyase from the phage LKA1 tailspike reduces Pseudomonas virulence. Sci Rep. 2017 Nov 24;7(1):16302. doi: 10.1038/s41598-017-16411-4. PMID:29176754 doi:https://dx.doi.org/10.1038/s41598-017-16411-4

[1]

@@ Line 1: / Line 1: @@
 ==Crystal Structure of the Pseudomonas phage phi297 tailspike gp61==
-<StructureSection load='4ru5' size='340' side='right' caption='[[4ru5]], [[Resolution|resolution]] 1.52&Aring;' scene=''>
+<StructureSection load='4ru5' size='340' side='right'caption='[[4ru5]], [[Resolution|resolution]] 1.52&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4ru5]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_bacteriophage_phi297 Pseudomonas aeruginosa bacteriophage phi297]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RU5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RU5 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4ru5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_phage_phi297 Pseudomonas phage phi297]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RU5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RU5 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">phi297_00061 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1129145 Pseudomonas aeruginosa bacteriophage phi297])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ru5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ru5 OCA], [https://pdbe.org/4ru5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ru5 RCSB], [https://www.ebi.ac.uk/pdbsum/4ru5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ru5 ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ru5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ru5 OCA], [http://pdbe.org/4ru5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ru5 RCSB], [http://www.ebi.ac.uk/pdbsum/4ru5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ru5 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/H2BD96_9CAUD H2BD96_9CAUD]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 17: / Line 18: @@
 </div>
 <div class="pdbe-citations 4ru5" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Tailspike protein|Tailspike protein]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Pseudomonas aeruginosa bacteriophage phi297]]
+[[Category: Large Structures]]
-[[Category: Browning, C]]
+[[Category: Pseudomonas phage phi297]]
-[[Category: Leiman, P G]]
+[[Category: Browning C]]
-[[Category: Shneider, M M]]
+[[Category: Leiman PG]]
-[[Category: Sycheva, L V]]
+[[Category: Shneider MM]]
-[[Category: Beta-helix]]
+[[Category: Sycheva LV]]
-[[Category: Lyase]]
-[[Category: Tailspike]]

4ru5: Difference between revisions

Revision as of 08:28, 8 March 2023

Crystal Structure of the Pseudomonas phage phi297 tailspike gp61

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Navigation menu

Page actions

Page actions

Personal tools

Navigation

Search

Tools