5gpy

Crystal structure of the human TFIIE complex

spinqualitylabelsall models 5gpy, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5gpy is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.1Å Ligands: ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function T2EA_HUMAN Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Publication Abstract from PubMed In eukaryotes, RNA polymerase II requires general transcription factors to initiate mRNA transcription. TFIIE subunits alpha and beta form a heterodimer and recruit TFIIH to complete the assembly of the pre-initiation complex. Here, we have determined the crystal structure of human TFIIE at atomic resolution. The N-terminal half of TFIIEalpha forms an extended winged helix (WH) domain with an additional helix, followed by a zinc-finger domain. TFIIEbeta contains the WH2 domain, followed by two coiled-coil helices intertwining with TFIIEalpha. We also showed that TFIIEalpha binds to TFIIEbeta with nanomolar affinity using isothermal titration calorimetry. In addition, mutations on the residues involved in the interactions resulted in severe growth defects in yeast. Lack of the C-terminal region of yeast TFIIEbeta causes a mild growth defect in vivo. These findings provide a structural basis for understanding the functional mechanisms of TFIIE in the context of pre-initiation complex formation and transcription initiation. Crystal Structure of Human General Transcription Factor TFIIE at Atomic Resolution.,Miwa K, Kojima R, Obita T, Ohkuma Y, Tamura Y, Mizuguchi M J Mol Biol. 2016 Oct 23;428(21):4258-4266. doi: 10.1016/j.jmb.2016.09.008. Epub, 2016 Sep 15. PMID:27639436 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Transcription initiation factors 3D structures

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