3tg3: Difference between revisions

Latest revision as of 17:31, 1 November 2023

Crystal structure of the MAPK binding domain of MKP7

Structural highlights

3tg3 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.675Å
Ligands:	EDO
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DUS16_HUMAN Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.^[1] ^[2]

Publication Abstract from PubMed

The mitogen-activated protein kinase (MAPK) cascades play a pivotal role in a myriad of cellular functions. The specificity and efficiency of MAPK signaling are controlled by docking interactions between MAPKs and their cognate proteins. Many MAPK-interacting partners, including substrates, MAPK kinases, phosphatases, and scaffolding proteins, have linear sequence motifs that mediate the interaction with the common docking site on MAPKs. We report the crystal structure of p38alpha in complex with the MAPK binding domain (KBD) from MAPK phosphatase 5 (MKP5) at 2.7 A resolution. In contrast to the well-known docking mode, the KBD binds p38alpha in a bipartite manner, in which two distinct helical regions of KBD engage the p38alpha docking site, which is situated on the back of the p38alpha active site. We also determined the crystal structure of the KBD of MKP7, which closely resembles the MKP5 KBD, suggesting that the mechanism of molecular recognition by the KBD of MKP5 is conserved in the cytoplasmic p38- and c-Jun N-terminal kinase-specific MKP subgroup. This previously unknown binding mode provides new insights into how MAPKs interact with their binding partners to achieve functional specificity.

A Distinct Interaction Mode Revealed by the Crystal Structure of the Kinase p38alpha with the MAPK Binding Domain of the Phosphatase MKP5.,Zhang YY, Wu JW, Wang ZX Sci Signal. 2011 Dec 20;4(204):ra88. PMID:22375048^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Masuda K, Shima H, Watanabe M, Kikuchi K. MKP-7, a novel mitogen-activated protein kinase phosphatase, functions as a shuttle protein. J Biol Chem. 2001 Oct 19;276(42):39002-11. Epub 2001 Aug 6. PMID:11489891 doi:https://dx.doi.org/10.1074/jbc.M104600200
↑ Willoughby EA, Collins MK. Dynamic interaction between the dual specificity phosphatase MKP7 and the JNK3 scaffold protein beta-arrestin 2. J Biol Chem. 2005 Jul 8;280(27):25651-8. Epub 2005 May 10. PMID:15888437 doi:https://dx.doi.org/10.1074/jbc.M501926200
↑ Zhang YY, Wu JW, Wang ZX. A Distinct Interaction Mode Revealed by the Crystal Structure of the Kinase p38alpha with the MAPK Binding Domain of the Phosphatase MKP5. Sci Signal. 2011 Dec 20;4(204):ra88. PMID:22375048 doi:10.1126/scisignal.2002241

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Masuda K, Shima H, Watanabe M, Kikuchi K. MKP-7, a novel mitogen-activated protein kinase phosphatase, functions as a shuttle protein. J Biol Chem. 2001 Oct 19;276(42):39002-11. Epub 2001 Aug 6. PMID:11489891 doi:https://dx.doi.org/10.1074/jbc.M104600200

[2] Willoughby EA, Collins MK. Dynamic interaction between the dual specificity phosphatase MKP7 and the JNK3 scaffold protein beta-arrestin 2. J Biol Chem. 2005 Jul 8;280(27):25651-8. Epub 2005 May 10. PMID:15888437 doi:https://dx.doi.org/10.1074/jbc.M501926200

[3] Zhang YY, Wu JW, Wang ZX. A Distinct Interaction Mode Revealed by the Crystal Structure of the Kinase p38alpha with the MAPK Binding Domain of the Phosphatase MKP5. Sci Signal. 2011 Dec 20;4(204):ra88. PMID:22375048 doi:10.1126/scisignal.2002241

[1]

[2]

[3]

@@ Line 3: / Line 3: @@
 <StructureSection load='3tg3' size='340' side='right'caption='[[3tg3]], [[Resolution|resolution]] 2.67&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3tg3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TG3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TG3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3tg3]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TG3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TG3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.675&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3tg1|3tg1]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DUSP16, KIAA1700, MKP7 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tg3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tg3 OCA], [https://pdbe.org/3tg3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tg3 RCSB], [https://www.ebi.ac.uk/pdbsum/3tg3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tg3 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/DUS16_HUMAN DUS16_HUMAN]] Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.<ref>PMID:11489891</ref> <ref>PMID:15888437</ref>
+[https://www.uniprot.org/uniprot/DUS16_HUMAN DUS16_HUMAN] Dual specificity protein phosphatase involved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.<ref>PMID:11489891</ref> <ref>PMID:15888437</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 27: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Liu, X]]
+[[Category: Liu X]]
-[[Category: Wang, Z X]]
+[[Category: Wang ZX]]
-[[Category: Wu, J W]]
+[[Category: Wu JW]]
-[[Category: Zhang, Y Y]]
+[[Category: Zhang YY]]
-[[Category: Hydrolase]]
-[[Category: Phosphatase]]
-[[Category: Rhodanese-like domain]]

3tg3: Difference between revisions

Latest revision as of 17:31, 1 November 2023

Crystal structure of the MAPK binding domain of MKP7

Structural highlights

Function

Publication Abstract from PubMed

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References

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