6lcj: Difference between revisions
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==TtGalA, alpha-galactosidase from Thermus thermopilus in apo form== | ==TtGalA, alpha-galactosidase from Thermus thermopilus in apo form== | ||
<StructureSection load='6lcj' size='340' side='right'caption='[[6lcj]]' scene=''> | <StructureSection load='6lcj' size='340' side='right'caption='[[6lcj]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LCJ FirstGlance]. <br> | <table><tr><td colspan='2'>[[6lcj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LCJ FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lcj OCA], [https://pdbe.org/6lcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lcj RCSB], [https://www.ebi.ac.uk/pdbsum/6lcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lcj ProSAT]</span></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lcj OCA], [https://pdbe.org/6lcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lcj RCSB], [https://www.ebi.ac.uk/pdbsum/6lcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lcj ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q53W51_THET8 Q53W51_THET8] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
alpha-Galactosidase catalyzes the hydrolysis of a terminal alpha-galactose residue in galacto-oligosaccharides and has potential in various industrial applications and food processing. We determined the crystal structures of alpha-galactosidase from the thermophilic microorganism Thermus thermophilus (TtGalA) and its complexes with pNPGal and stachyose. The monomer folds into an N-terminal domain, a catalytic (beta/alpha)8 barrel domain, and a C-terminal domain. The domain organization is similar to that of the other family of 36 alpha-galactosidases, but TtGalA presents a cagelike hexamer. Structural analysis shows that oligomerization may be a key factor for the thermal adaption of TtGalA. The structure of TtGalA complexed with stachyose reveals only the existence of one -1 subsite and one +1 subsite in the active site. Structural comparison of the stachyose-bound complexes of TtGalA and GsAgaA, a tetrameric enzyme with four subsites, suggests evolutionary divergence of substrate specificity within the GH36 family of alpha-galactosidases. To the best of our knowledge, the crystal structure of TtGalA is the first report of a quaternary structure as a hexameric assembly in the alpha-galactosidase family. | |||
Crystal Structure of alpha-Galactosidase from Thermus thermophilus: Insight into Hexamer Assembly and Substrate Specificity.,Chen SC, Wu SP, Chang YY, Hwang TS, Lee TH, Hsu CH J Agric Food Chem. 2020 Jun 3;68(22):6161-6169. doi: 10.1021/acs.jafc.0c00871., Epub 2020 May 20. PMID:32390413<ref>PMID:32390413</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6lcj" style="background-color:#fffaf0;"></div> | |||
==See Also== | ==See Also== | ||
*[[Galactosidase 3D structures|Galactosidase 3D structures]] | *[[Galactosidase 3D structures|Galactosidase 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Thermus thermophilus HB8]] | |||
[[Category: Chen SC]] | [[Category: Chen SC]] | ||
[[Category: Hsu CH]] | [[Category: Hsu CH]] | ||
Latest revision as of 10:56, 22 November 2023
TtGalA, alpha-galactosidase from Thermus thermopilus in apo form
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