2px3: Difference between revisions

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<StructureSection load='2px3' size='340' side='right'caption='[[2px3]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='2px3' size='340' side='right'caption='[[2px3]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2px3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PX3 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2px3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PX3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2px0|2px0]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">flhF ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2px3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2px3 OCA], [https://pdbe.org/2px3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2px3 RCSB], [https://www.ebi.ac.uk/pdbsum/2px3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2px3 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2px3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2px3 OCA], [https://pdbe.org/2px3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2px3 RCSB], [https://www.ebi.ac.uk/pdbsum/2px3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2px3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU]] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.  
[https://www.uniprot.org/uniprot/FLHF_BACSU FLHF_BACSU] Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2px3 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2px3 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Flagella are well characterized as the organelles of locomotion and allow bacteria to react to environmental changes. The assembly of flagella is a multistep process and relies on a complex type III export machinery located in the cytoplasmic membrane. The FlhF protein is essential for the placement and assembly of polar flagella and has been classified as a signal-recognition particle (SRP)-type GTPase. SRP GTPases appeared early in evolution and form a unique subfamily within the guanine nucleotide binding proteins with only three members: the signal sequence-binding protein SRP54, the SRP receptor FtsY, and FlhF. We report the crystal structures of FlhF from Bacillus subtilis in complex with GTP and GMPPNP. FlhF shares SRP GTPase-specific features such as the presence of an N-terminal alpha-helical domain and the I-box insertion. It forms a symmetric homodimer sequestering a composite active site that contains two head-to-tail arranged nucleotides similar to the heterodimeric SRP-targeting complex. However, significant differences to the GTPases of SRP and the SRP receptor include the formation of a stable homodimer with GTP as well as severe modifications and even the absence of motifs involved in regulation of the other two SRP GTPases. Our results provide insights into SRP GTPases and their roles in two fundamentally different protein-targeting routes that both rely on efficient protein delivery to a secretion channel.
The crystal structure of the third signal-recognition particle GTPase FlhF reveals a homodimer with bound GTP.,Bange G, Petzold G, Wild K, Parlitz RO, Sinning I Proc Natl Acad Sci U S A. 2007 Aug 21;104(34):13621-5. Epub 2007 Aug 15. PMID:17699634<ref>PMID:17699634</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2px3" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
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*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
*[[GTP-binding protein 3D structures|GTP-binding protein 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Vibrio subtilis ehrenberg 1835]]
[[Category: Bacillus subtilis]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Bange, G]]
[[Category: Bange G]]
[[Category: Sinning, I]]
[[Category: Sinning I]]
[[Category: Wild, K]]
[[Category: Wild K]]
[[Category: Biosynthetic protein]]
[[Category: Flagellum]]
[[Category: Protein transport]]
[[Category: Srp gtpase]]

Latest revision as of 09:12, 21 February 2024

Crystal structure of FlhF complexed with GTP/Mg(2+)

2px3, resolution 3.20Å

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