3i1a: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3i1a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_serogroup_1 Legionella pneumophila serogroup 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I1A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I1A FirstGlance]. <br>
<table><tr><td colspan='2'>[[3i1a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_serogroup_1 Legionella pneumophila serogroup 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3I1A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3I1A FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3i0o|3i0o]], [[3i0q|3i0q]]</div></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMS:DIMETHYL+SULFOXIDE'>DMS</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aph, aph9 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=66976 Legionella pneumophila serogroup 1])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i1a OCA], [https://pdbe.org/3i1a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i1a RCSB], [https://www.ebi.ac.uk/pdbsum/3i1a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i1a ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3i1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3i1a OCA], [https://pdbe.org/3i1a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3i1a RCSB], [https://www.ebi.ac.uk/pdbsum/3i1a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3i1a ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O06916_LEGPN O06916_LEGPN]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i1a ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3i1a ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Aminoglycoside phosphotransferases (APHs) constitute a diverse group of enzymes that are often the underlying cause of aminoglycoside resistance in the clinical setting. Several APHs have been extensively characterized, including the elucidation of the three-dimensional structure of two APH(3') isozymes and an APH(2'') enzyme. Although many APHs are plasmid-encoded and are capable of inactivating numerous 2-deoxystreptmaine aminoglycosides with multiple regiospecificity, APH(9)-Ia, isolated from Legionella pneumophila, is an unusual enzyme among the APH family for its chromosomal origin and its specificity for a single non-2-deoxystreptamine aminoglycoside substrate, spectinomycin. We describe here the crystal structures of APH(9)-Ia in its apo form, its binary complex with the nucleotide, AMP, and its ternary complex bound with ADP and spectinomycin. The structures reveal that APH(9)-Ia adopts the bilobal protein kinase-fold, analogous to the APH(3') and APH(2'') enzymes. However, APH(9)-Ia differs significantly from the other two types of APH enzymes in its substrate binding area and that it undergoes a conformation change upon ligand binding. Moreover, kinetic assay experiments indicate that APH(9)-Ia has stringent substrate specificity as it is unable to phosphorylate substrates of choline kinase or methylthioribose kinase despite high structural resemblance. The crystal structures of APH(9)-Ia demonstrate and expand our understanding of the diversity of the APH family, which in turn will facilitate the development of new antibiotics and inhibitors.
Structure of the antibiotic resistance factor spectinomycin phosphotransferase from Legionella pneumophila.,Fong DH, Lemke CT, Hwang J, Xiong B, Berghuis AM J Biol Chem. 2010 Mar 26;285(13):9545-55. Epub 2010 Jan 19. PMID:20089863<ref>PMID:20089863</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3i1a" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Legionella pneumophila serogroup 1]]
[[Category: Legionella pneumophila serogroup 1]]
[[Category: Berghuis, A M]]
[[Category: Berghuis AM]]
[[Category: Fong, D H]]
[[Category: Fong DH]]
[[Category: Hwang, J]]
[[Category: Hwang J]]
[[Category: Lemke, C T]]
[[Category: Lemke CT]]
[[Category: Xiong, B]]
[[Category: Xiong B]]
[[Category: Aminoglycoside phosphotransferase]]
[[Category: Antibiotic resistance]]
[[Category: Protein kinase]]
[[Category: Transferase]]

Latest revision as of 10:02, 21 February 2024

Crystal Structure of apo Spectinomycin Phosphotransferase, APH(9)-Ia

3i1a, resolution 1.70Å

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