4k7r: Difference between revisions

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4k7r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K7R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K7R FirstGlance]. <br>
<table><tr><td colspan='2'>[[4k7r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4K7R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4K7R FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PK:(2S)-1-(PENTANOYLOXY)PROPAN-2-YL+HEXANOATE'>3PK</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.094&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PK:(2S)-1-(PENTANOYLOXY)PROPAN-2-YL+HEXANOATE'>3PK</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k7r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k7r OCA], [https://pdbe.org/4k7r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k7r RCSB], [https://www.ebi.ac.uk/pdbsum/4k7r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k7r ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4k7r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4k7r OCA], [https://pdbe.org/4k7r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4k7r RCSB], [https://www.ebi.ac.uk/pdbsum/4k7r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4k7r ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[https://www.uniprot.org/uniprot/CUSC_ECOLI CUSC_ECOLI] Part of a cation efflux system that mediates resistance to copper and silver. In pathogenic strains it allows the bacteria to invade brain microvascular endothelial cells (BMEC) thus allowing it to cross the blood-brain barrier and cause neonatal meningitis.<ref>PMID:11399769</ref> <ref>PMID:12813074</ref>  
[https://www.uniprot.org/uniprot/CUSC_ECOLI CUSC_ECOLI] Part of a cation efflux system that mediates resistance to copper and silver. In pathogenic strains it allows the bacteria to invade brain microvascular endothelial cells (BMEC) thus allowing it to cross the blood-brain barrier and cause neonatal meningitis.<ref>PMID:11399769</ref> <ref>PMID:12813074</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Gram-negative bacteria, such as Escherichia coli, frequently utilize tripartite efflux complexes in the RND (resistance-nodulation-cell division) family to expel diverse toxic compounds from the cell. These complexes span both the inner and outer membranes of the bacterium via an alpha-helical, inner membrane transporter; a periplasmic membrane fusion protein; and a beta-barrel, outer membrane channel. One such efflux system, CusCBA, is responsible for extruding biocidal Cu(I) and Ag(I) ions. To remove these toxic ions, the CusC outer membrane channel must form a beta-barrel structural domain, which creates a pore and spans the entire outer membrane. We here report the crystal structures of wild-type CusC, as well as two CusC mutants, suggesting that the first N-terminal cysteine residue plays an important role in protein-membrane interactions and is critical for the insertion of this channel protein into the outer membrane. These structures provide insight into the mechanisms on CusC folding and transmembrane channel formation. It is also found that the interactions between CusC and membrane may be crucial for controlling the opening and closing of this beta-barrel, outer membrane channel.
Crystal Structures of CusC Review Conformational Changes Accompanying Folding and Transmembrane Channel Formation.,Lei HT, Bolla JR, Su CC, Yu EW J Mol Biol. 2013 Oct 4. pii: S0022-2836(13)00626-8. doi:, 10.1016/j.jmb.2013.09.042. PMID:24099674<ref>PMID:24099674</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4k7r" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>

Latest revision as of 12:09, 1 March 2024

Crystal structures of CusC review conformational changes accompanying folding and transmembrane channel formation

4k7r, resolution 2.09Å

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