3sz7: Difference between revisions

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<StructureSection load='3sz7' size='340' side='right'caption='[[3sz7]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
<StructureSection load='3sz7' size='340' side='right'caption='[[3sz7]], [[Resolution|resolution]] 1.72&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3sz7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspfu Aspfu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SZ7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[3sz7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus_Af293 Aspergillus fumigatus Af293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SZ7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SZ7 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AFUA_1G09830 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=330879 ASPFU])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.72&#8491;</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phosphoprotein_phosphatase Phosphoprotein phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.16 3.1.3.16] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sz7 OCA], [https://pdbe.org/3sz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sz7 RCSB], [https://www.ebi.ac.uk/pdbsum/3sz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sz7 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sz7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sz7 OCA], [https://pdbe.org/3sz7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sz7 RCSB], [https://www.ebi.ac.uk/pdbsum/3sz7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sz7 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q4WTC0_ASPFU Q4WTC0_ASPFU]
The insertion of tail-anchored transmembrane (TA) proteins into the appropriate membrane is a post-translational event that requires stabilization of the transmembrane domain and targeting to the proper destination. Sgt2 is a heat-shock protein cognate (HSC) co-chaperone that preferentially binds endoplasmic reticulum-destined TA proteins and directs them to the GET pathway via Get4 and Get5. Here, we present the crystal structure from a fungal Sgt2 homolog of the tetratrico-repeat (TPR) domain and part of the linker that connects to the C-terminal domain. The linker extends into the two-carboxylate clamp of the TPR domain from a symmetry-related molecule mimicking the binding to HSCs. Based on this structure, we provide biochemical evidence that the Sgt2 TPR domain has the ability to directly bind multiple HSC family members. The structure allows us to propose features involved in this lower specificity relative to other TPR containing co-chaperones. We further show that a dimer of Sgt2 binds a single Get5 and use small angle x-ray scattering to characterize the domain arrangement of Sgt2 in solution. These results allow us to present a structural model of the Sgt2-Get4/Get5-HSC complex.
 
A structural model of the Sgt2 protein and its interactions with chaperones and the Get4/Get5 complex.,Chartron JW, Gonzalez GM, Clemons WM Jr J Biol Chem. 2011 Sep 30;286(39):34325-34. Epub 2011 Aug 10. PMID:21832041<ref>PMID:21832041</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3sz7" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Small glutamine-rich tetratricopeptide repeat containing protein alpha|Small glutamine-rich tetratricopeptide repeat containing protein alpha]]
*[[Small glutamine-rich tetratricopeptide repeat containing protein alpha|Small glutamine-rich tetratricopeptide repeat containing protein alpha]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aspfu]]
[[Category: Aspergillus fumigatus Af293]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Phosphoprotein phosphatase]]
[[Category: Chartron JW]]
[[Category: Chartron, J W]]
[[Category: Clemons Jr WM]]
[[Category: Clemons, W M]]
[[Category: Gonzalez GM]]
[[Category: Gonzalez, G M]]
[[Category: Chaperone regulator]]
[[Category: Cochaperone]]
[[Category: Get3]]
[[Category: Get4]]
[[Category: Get5]]
[[Category: Hsc82]]
[[Category: Hsp104]]
[[Category: Mdy2]]
[[Category: Ssa1]]
[[Category: Sse1]]
[[Category: Tpr domain]]

Latest revision as of 13:12, 14 March 2024

Crystal structure of the Sgt2 TPR domain from Aspergillus fumigatus

3sz7, resolution 1.72Å

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