6a9w: Difference between revisions

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<StructureSection load='6a9w' size='340' side='right'caption='[[6a9w]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='6a9w' size='340' side='right'caption='[[6a9w]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[6a9w]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A9W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A9W FirstGlance]. <br>
<table><tr><td colspan='2'>[[6a9w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitratiruptor_phage_NrS-1 Nitratiruptor phage NrS-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A9W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A9W FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a9w OCA], [http://pdbe.org/6a9w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a9w RCSB], [http://www.ebi.ac.uk/pdbsum/6a9w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a9w ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a9w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a9w OCA], [https://pdbe.org/6a9w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a9w RCSB], [https://www.ebi.ac.uk/pdbsum/6a9w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a9w ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/M5AAG8_9CAUD M5AAG8_9CAUD]
A novel DNA polymerase found in the deep-sea vent phage NrS-1, was confirmed to have both DNA polymerase and primase activities. In this polymerase, the N-terminal residues 1-300 (referred to as N300) are the core region required for polymerizing DNA and catalyzing de novo DNA synthesis. Here, the crystal structure of N300 was solved at a resolution of 1.80A. The overall structure consists of a prim/pol domain and a helix bundle domain, which are separated by a 14-residue-long flexible tether (residues 177-190). Both the prim/pol domain of N300 and other primase-polymerases (prim-pol) encompass an analogous fold with conserved catalytic residues. Mutagenesis and enzymatic activity assays show that the acidic active-site residue E139 is required for both polymerase and primase activities. Functional assays confirm the essentiality of the helix bundle domain for primase activity. Furthermore, we identified a mutant (N300-Y261A) of the helix bundle domain, which probably plays an indispensable role in the primer initiation and recognition of template DNA.


Crystal structure and biochemical studies of the bifunctional DNA primase-polymerase from phage NrS-1.,Guo H, Li M, Wang T, Wu H, Zhou H, Xu C, Yu F, Liu X, He J Biochem Biophys Res Commun. 2019 Mar 19;510(4):573-579. doi:, 10.1016/j.bbrc.2019.01.144. Epub 2019 Feb 7. PMID:30739783<ref>PMID:30739783</ref>
==See Also==
 
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6a9w" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Guo, H J]]
[[Category: Nitratiruptor phage NrS-1]]
[[Category: He, J H]]
[[Category: Guo HJ]]
[[Category: Li, M J]]
[[Category: He JH]]
[[Category: Liu, X P]]
[[Category: Li MJ]]
[[Category: Wang, T L]]
[[Category: Liu XP]]
[[Category: Wu, H]]
[[Category: Wang TL]]
[[Category: Xu, C Y]]
[[Category: Wu H]]
[[Category: Yu, F]]
[[Category: Xu CY]]
[[Category: Zhou, H]]
[[Category: Yu F]]
[[Category: Prim-pol]]
[[Category: Zhou H]]
[[Category: Replication]]

Latest revision as of 10:31, 27 March 2024

Structure of the bifunctional DNA primase-polymerase from phage NrS-1

6a9w, resolution 1.80Å

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