3qpt: Difference between revisions

Latest revision as of 09:36, 30 October 2024

Crystal structure of the Salmonella transcriptional regulator SlyA

Structural highlights

3qpt is a 1 chain structure with sequence from Salmonella enterica subsp. enterica serovar Typhimurium. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.4Å
Ligands:	MSE
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SLYA_SALTY Transcription regulator that can specifically activate or repress expression of target genes. Required for virulence and survival in the macrophage environment. Probably activates expression of ispA, xseB genes, and of omp operon.^[1] ^[2]

Publication Abstract from PubMed

SlyA is a master virulence regulator that controls the transcription of numerous genes in Salmonella enterica. We present here crystal structures of SlyA by itself and bound to a high-affinity DNA operator sequence in the slyA gene. SlyA interacts with DNA through direct recognition of a guanine base by R65, as well as interactions between conserved R86 and the minor groove and a large network of non-base-specific contacts to the sugar-phosphate backbone. Our structures, together with an unpublished structure of SlyA bound to the drug salicylate (PDB: 3DEU), reveal that unlike many other MarR-family proteins, SlyA dissociates from DNA without large conformational changes when bound to this effector. We propose that SlyA and other MarR global regulators rely more on indirect readout of DNA sequence in order to exert control over many genes, in contrast to proteins such as OhrR which recognize a single operator.

Crystal structures of SlyA, a master virulence regulator of Salmonella, in free and DNA-bound states.,Dolan KT, Duguid EM, He C J Biol Chem. 2011 May 5. PMID:21550983^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ludwig A, Tengel C, Bauer S, Bubert A, Benz R, Mollenkopf HJ, Goebel W. SlyA, a regulatory protein from Salmonella typhimurium, induces a haemolytic and pore-forming protein in Escherichia coli. Mol Gen Genet. 1995 Dec 15;249(5):474-86. PMID:8544813
↑ Buchmeier N, Bossie S, Chen CY, Fang FC, Guiney DG, Libby SJ. SlyA, a transcriptional regulator of Salmonella typhimurium, is required for resistance to oxidative stress and is expressed in the intracellular environment of macrophages. Infect Immun. 1997 Sep;65(9):3725-30. PMID:9284144
↑ Dolan KT, Duguid EM, He C. Crystal structures of SlyA, a master virulence regulator of Salmonella, in free and DNA-bound states. J Biol Chem. 2011 May 5. PMID:21550983 doi:10.1074/jbc.M111.245258

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OCA

[1] Ludwig A, Tengel C, Bauer S, Bubert A, Benz R, Mollenkopf HJ, Goebel W. SlyA, a regulatory protein from Salmonella typhimurium, induces a haemolytic and pore-forming protein in Escherichia coli. Mol Gen Genet. 1995 Dec 15;249(5):474-86. PMID:8544813

[2] Buchmeier N, Bossie S, Chen CY, Fang FC, Guiney DG, Libby SJ. SlyA, a transcriptional regulator of Salmonella typhimurium, is required for resistance to oxidative stress and is expressed in the intracellular environment of macrophages. Infect Immun. 1997 Sep;65(9):3725-30. PMID:9284144

[3] Dolan KT, Duguid EM, He C. Crystal structures of SlyA, a master virulence regulator of Salmonella, in free and DNA-bound states. J Biol Chem. 2011 May 5. PMID:21550983 doi:10.1074/jbc.M111.245258

[1]

[2]

[3]

@@ Line 3: / Line 3: @@
 <StructureSection load='3qpt' size='340' side='right'caption='[[3qpt]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3qpt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_typhimurium"_loeffler_1892 "bacillus typhimurium" loeffler 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QPT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3qpt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QPT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QPT FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3q5f|3q5f]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">slyA, STM1444 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90371 "Bacillus typhimurium" Loeffler 1892])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qpt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qpt OCA], [https://pdbe.org/3qpt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qpt RCSB], [https://www.ebi.ac.uk/pdbsum/3qpt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qpt ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/SLYA_SALTY SLYA_SALTY]] Transcription regulator that can specifically activate or repress expression of target genes. Required for virulence and survival in the macrophage environment. Probably activates expression of ispA, xseB genes, and of omp operon.<ref>PMID:8544813</ref> <ref>PMID:9284144</ref>
+[https://www.uniprot.org/uniprot/SLYA_SALTY SLYA_SALTY] Transcription regulator that can specifically activate or repress expression of target genes. Required for virulence and survival in the macrophage environment. Probably activates expression of ispA, xseB genes, and of omp operon.<ref>PMID:8544813</ref> <ref>PMID:9284144</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 27: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus typhimurium loeffler 1892]]
 [[Category: Large Structures]]
-[[Category: Dolan, K T]]
+[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
-[[Category: Duguid, E M]]
+[[Category: Dolan KT]]
-[[Category: Marr/slya protein family]]
+[[Category: Duguid EM]]
-[[Category: Transcription]]
-[[Category: Transcriptonal regulator]]
-[[Category: Winged helix-turn-helix]]

3qpt: Difference between revisions

Latest revision as of 09:36, 30 October 2024

Crystal structure of the Salmonella transcriptional regulator SlyA

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

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