9im9: Difference between revisions
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==CTB10-M4-(S)-1d complex== | |||
<StructureSection load='9im9' size='340' side='right'caption='[[9im9]], [[Resolution|resolution]] 1.81Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9im9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cercospora_sp._JNU001 Cercospora sp. JNU001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9IM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9IM9 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.81Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A1D9Q:(3~{S})-1,1-bis(chloranyl)-5-methyl-spiro[1~{H}-indole-3,2-cyclopropane]-2-one'>A1D9Q</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PBF:PARA-(BENZOYL)-PHENYLALANINE'>PBF</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9im9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9im9 OCA], [https://pdbe.org/9im9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9im9 RCSB], [https://www.ebi.ac.uk/pdbsum/9im9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9im9 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/A0A977K7H6_9PEZI A0A977K7H6_9PEZI] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The creation of enzymes with abiological abilities offers exciting opportunities to access new-to-nature biocatalysis beyond that found in nature. Here, we repurpose a novel protein scaffold, CTB10, as an artificial photoenzyme through genetic code expansion. It enables catalytic deracemization of cyclopropane, a process that remains inaccessible to traditional biocatalysis due to its thermodynamically unfavorable nature. Following structural optimization through directed evolution, a broad substrate scope with high enantioselectivities is achieved. Furthermore, the crystal structure of the CTB10-based photoenzyme-substrate complex well demonstrates how the catalytic chiral cavity is sculpted to promote efficient and selective light-enabled deracemization. Therefore, this study unlocks the potential for achieving challenging deracemization through biocatalysis. | |||
Light-Driven Deracemization by a Designed Photoenzyme.,Li M, Zhang Y, Fu K, Deng Z, Yuan Z, Luo Z, Rao Y J Am Chem Soc. 2025 Apr 23;147(16):13190-13199. doi: 10.1021/jacs.4c16521. Epub , 2025 Apr 12. PMID:40219972<ref>PMID:40219972</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 9im9" style="background-color:#fffaf0;"></div> | ||
[[Category: Fu | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cercospora sp. JNU001]] | |||
[[Category: Large Structures]] | |||
[[Category: Fu K]] | |||
[[Category: Rao YJ]] | |||
