9s1f: Difference between revisions
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==Cryo-EM structure of activated retron Eco2 (Ec67)== | |||
<StructureSection load='9s1f' size='340' side='right'caption='[[9s1f]], [[Resolution|resolution]] 2.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[9s1f]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_NCTC_86 Escherichia coli NCTC 86]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9S1F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9S1F FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9Å</td></tr> | |||
[[Category: | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9s1f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9s1f OCA], [https://pdbe.org/9s1f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9s1f RCSB], [https://www.ebi.ac.uk/pdbsum/9s1f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9s1f ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RT67_ECOLX RT67_ECOLX] Reverse transcriptase (RT) component of antiviral defense system retron Ec67, minimally composed of a non-coding RNA (ncRNA) and this RT. Expression of these 2 elements confers protection against bacteriophage T5. At multiplicity of infection (MOI) of 0.02 cultures grow normally when infected with T5 without collapsing, at MOI 2 cultures enter growth stasis (PubMed:33157039). Responsible for synthesis of msDNA-Ec67 (a branched molecule with RNA linked by a 2',5'-phosphodiester bond to ssDNA). The retron transcript serves as primer (from a conserved internal G residue) and template for the reaction, and codes for the RT (PubMed:1378431, PubMed:1692831, PubMed:2466332). Can use other retrons as substrate (msDNA-Mx162 and msDNA-Ec86). Also able to synthesize DNA from a DNA template at least in vitro, although the enzyme is less active with a DNA template (PubMed:1692831).<ref>PMID:1378431</ref> <ref>PMID:1692831</ref> <ref>PMID:2466332</ref> <ref>PMID:33157039</ref> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Escherichia coli NCTC 86]] | |||
[[Category: Large Structures]] | |||
[[Category: Grigaitis R]] | |||
[[Category: Jasnauskaite M]] | |||
[[Category: Malinauskaite L]] | |||
[[Category: Pausch P]] | |||
[[Category: Skorupskaite A]] | |||
Latest revision as of 07:17, 18 February 2026
Cryo-EM structure of activated retron Eco2 (Ec67)
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