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====
==In situ structure of nebulin bound to actin filament in skeletal sarcomere==
<StructureSection load='7qim' size='340' side='right'caption='[[7qim]]' scene=''>
<StructureSection load='7qim' size='340' side='right'caption='[[7qim]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
<table><tr><td colspan='2'>[[7qim]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QIM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QIM FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qim OCA], [https://pdbe.org/7qim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qim RCSB], [https://www.ebi.ac.uk/pdbsum/7qim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qim ProSAT]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qim OCA], [https://pdbe.org/7qim PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qim RCSB], [https://www.ebi.ac.uk/pdbsum/7qim PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qim ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ACTS_MOUSE ACTS_MOUSE] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In skeletal muscle, nebulin stabilizes and regulates the length of thin filaments, but the underlying mechanism remains nebulous. In this work, we used cryo-electron tomography and subtomogram averaging to reveal structures of native nebulin bound to thin filaments within intact sarcomeres. This in situ reconstruction provided high-resolution details of the interaction between nebulin and actin, demonstrating the stabilizing role of nebulin. Myosin bound to the thin filaments exhibited different conformations of the neck domain, highlighting its inherent structural variability in muscle. Unexpectedly, nebulin did not interact with myosin or tropomyosin, but it did interact with a troponin T linker through two potential binding motifs on nebulin, explaining its regulatory role. Our structures support the role of nebulin as a thin filament "molecular ruler" and provide a molecular basis for studying nemaline myopathies.
Structures from intact myofibrils reveal mechanism of thin filament regulation through nebulin.,Wang Z, Grange M, Pospich S, Wagner T, Kho AL, Gautel M, Raunser S Science. 2022 Feb 18;375(6582):eabn1934. doi: 10.1126/science.abn1934. Epub 2022 , Feb 18. PMID:35175800<ref>PMID:35175800</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 7qim" style="background-color:#fffaf0;"></div>
==See Also==
*[[Nebulin|Nebulin]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Z-disk]]
[[Category: Mus musculus]]
[[Category: Gautel M]]
[[Category: Grange M]]
[[Category: Kho AL]]
[[Category: Pospich S]]
[[Category: Raunser S]]
[[Category: Wagner T]]
[[Category: Wang Z]]
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