7zg5: Difference between revisions
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== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/ | [https://www.uniprot.org/uniprot/TACT3_SALT1 TACT3_SALT1] Toxic component of a type II toxin-antitoxin (TA) system (PubMed:29777131, PubMed:34556858). Acetylates tRNA and inhibits translation (PubMed:29777131). Acetylates only Gly-tRNA on all 3 Gly-tRNA(Gly) isoacceptors in situ (PubMed:35609997). In vitro acetylates mainly Ile/Leu and Gly (PubMed:29777131). Overexpression during the lag phase of a tacA3-tacT3 deletion strain leads to a 150-fold increase in persister cells in the presence of cefotaxime and a non-growth state in the absence of antibiotic (PubMed:29777131). Persister cell formation and the growth defect are neutralized by cognate antitoxin TacA3, but not by TacA1 or TacA2 (PubMed:29777131, PubMed:34556858). Plays a role in persister cell formation (PubMed:24408438).<ref>PMID:24408438</ref> <ref>PMID:29777131</ref> <ref>PMID:34556858</ref> <ref>PMID:35609997</ref> The TacA3-TacT3 complex both represses and derepresses expression of its own operon (PubMed:38538913). The hexameric 4:2 TacA3-TacT3 complex binds promoter DNA and represses its transcription; both subunits are required (PubMed:38538913). The octomeric 4:4 TacA3-TacT3 complex derepresses the operon (PubMed:38538913). The shift from hexameric to octomeric complex probably alters DNA-binding, leading to dissociation from the operator DNA and derepression (Probable) (PubMed:38538913).<ref>PMID:38538913</ref> <ref>PMID:38538913</ref> | ||
== References == | |||
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</StructureSection> | </StructureSection> | ||
