21ft: Difference between revisions

Latest revision as of 06:35, 3 June 2026

GABA aminotransferase from Arabidopsis thaliana

Structural highlights

21ft is a 4 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:	LLP
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GATP_ARATH Transaminase that degrades gamma-amino butyric acid (GABA) and uses pyruvate or glyoxylate as amino-group acceptor, but not 2-oxoglutarate. The pyruvate-dependent activity is reversible while the glyoxylate-dependent activity is irreversible. Cannot use beta-alanine, ornithine, acetylornithine, serine, glycine, asparagine, glutamine, glutamate, valine, leucine, isoleucine, methionine, phenylalanine, histidine, lysine, arginine, aspartate, threonine, tyrosine, tryptophan, proline, or cysteine as amino donors. Modulates steady-state GABA levels in diploid pistil cells and the haploid pollen tube. Involved in the formation of a gradient of GABA along the pollen tube path. Involved in the maintenance of the shoot apical meristem (SAM) structure and subsequent adaxial-abaxial axis-dependent development of cotyledons and leaves (PubMed:21690177).^[1] ^[2] ^[3] ^[4] [UniProtKB:P12995]

References

↑ Palanivelu R, Brass L, Edlund AF, Preuss D. Pollen tube growth and guidance is regulated by POP2, an Arabidopsis gene that controls GABA levels. Cell. 2003 Jul 11;114(1):47-59. doi: 10.1016/s0092-8674(03)00479-3. PMID:12859897 doi:https://dx.doi.org/10.1016/s0092-8674(03)00479-3
↑ Mirabella R, Rauwerda H, Struys EA, Jakobs C, Triantaphylidès C, Haring MA, Schuurink RC. The Arabidopsis her1 mutant implicates GABA in E-2-hexenal responsiveness. Plant J. 2008 Jan;53(2):197-213. PMID:17971036 doi:10.1111/j.1365-313X.2007.03323.x
↑ Clark SM, Di Leo R, Dhanoa PK, Van Cauwenberghe OR, Mullen RT, Shelp BJ. Biochemical characterization, mitochondrial localization, expression, and potential functions for an Arabidopsis gamma-aminobutyrate transaminase that utilizes both pyruvate and glyoxylate. J Exp Bot. 2009;60(6):1743-57. PMID:19264755 doi:10.1093/jxb/erp044
↑ Toyokura K, Watanabe K, Oiwaka A, Kusano M, Tameshige T, Tatematsu K, Matsumoto N, Tsugeki R, Saito K, Okada K. Succinic semialdehyde dehydrogenase is involved in the robust patterning of Arabidopsis leaves along the adaxial-abaxial axis. Plant Cell Physiol. 2011 Aug;52(8):1340-53. PMID:21690177 doi:10.1093/pcp/pcr079

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OCA

[1] Palanivelu R, Brass L, Edlund AF, Preuss D. Pollen tube growth and guidance is regulated by POP2, an Arabidopsis gene that controls GABA levels. Cell. 2003 Jul 11;114(1):47-59. doi: 10.1016/s0092-8674(03)00479-3. PMID:12859897 doi:https://dx.doi.org/10.1016/s0092-8674(03)00479-3

[2] Mirabella R, Rauwerda H, Struys EA, Jakobs C, Triantaphylidès C, Haring MA, Schuurink RC. The Arabidopsis her1 mutant implicates GABA in E-2-hexenal responsiveness. Plant J. 2008 Jan;53(2):197-213. PMID:17971036 doi:10.1111/j.1365-313X.2007.03323.x

[3] Clark SM, Di Leo R, Dhanoa PK, Van Cauwenberghe OR, Mullen RT, Shelp BJ. Biochemical characterization, mitochondrial localization, expression, and potential functions for an Arabidopsis gamma-aminobutyrate transaminase that utilizes both pyruvate and glyoxylate. J Exp Bot. 2009;60(6):1743-57. PMID:19264755 doi:10.1093/jxb/erp044

[4] Toyokura K, Watanabe K, Oiwaka A, Kusano M, Tameshige T, Tatematsu K, Matsumoto N, Tsugeki R, Saito K, Okada K. Succinic semialdehyde dehydrogenase is involved in the robust patterning of Arabidopsis leaves along the adaxial-abaxial axis. Plant Cell Physiol. 2011 Aug;52(8):1340-53. PMID:21690177 doi:10.1093/pcp/pcr079

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@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 21ft is ON HOLD  until Paper Publication
+==GABA aminotransferase from Arabidopsis thaliana==
+<StructureSection load='21ft' size='340' side='right'caption='[[21ft]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[21ft]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=21FT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=21FT FirstGlance]. <br>
-Description:
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=21ft FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=21ft OCA], [https://pdbe.org/21ft PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=21ft RCSB], [https://www.ebi.ac.uk/pdbsum/21ft PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=21ft ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/GATP_ARATH GATP_ARATH] Transaminase that degrades gamma-amino butyric acid (GABA) and uses pyruvate or glyoxylate as amino-group acceptor, but not 2-oxoglutarate. The pyruvate-dependent activity is reversible while the glyoxylate-dependent activity is irreversible. Cannot use beta-alanine, ornithine, acetylornithine, serine, glycine, asparagine, glutamine, glutamate, valine, leucine, isoleucine, methionine, phenylalanine, histidine, lysine, arginine, aspartate, threonine, tyrosine, tryptophan, proline, or cysteine as amino donors. Modulates steady-state GABA levels in diploid pistil cells and the haploid pollen tube. Involved in the formation of a gradient of GABA along the pollen tube path. Involved in the maintenance of the shoot apical meristem (SAM) structure and subsequent adaxial-abaxial axis-dependent development of cotyledons and leaves (PubMed:21690177).<ref>PMID:12859897</ref> <ref>PMID:17971036</ref> <ref>PMID:19264755</ref> <ref>PMID:21690177</ref> [UniProtKB:P12995]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Arabidopsis thaliana]]
+[[Category: Large Structures]]
+[[Category: Kobata K]]
+[[Category: Nagata K]]
+[[Category: Okoda N]]
+[[Category: Okuda S]]
+[[Category: Sano K]]
+[[Category: Tsutsumi K]]

21ft: Difference between revisions

Latest revision as of 06:35, 3 June 2026

GABA aminotransferase from Arabidopsis thaliana

Structural highlights

Function

References

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