3syn

Crystal structure of FlhF in complex with its activator

spinqualitylabelsall models 3syn, resolution 3.06Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3syn is a 8 chain structure with sequence from Bacillus subtilis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.063Å Ligands: AF3, GDP, MG, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FLHF_BACSU Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum. Publication Abstract from PubMed Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviate from the canonical switch paradigm in that no GAPs have been identified. Here we show that the YlxH protein activates the SRP-GTPase FlhF. The crystal structure of the Bacillus subtilis FlhF-effector complex revealed that the effector does not contribute a catalytic residue but positions the catalytic machinery already present in SRP-GTPases. We provide a general concept that might also apply to the RNA-driven activation of the universally conserved, co-translational protein-targeting machinery comprising the SRP-GTPases Ffh and FtsY. Our study exemplifies the evolutionary transition from RNA- to protein-driven activation in SRP-GTPases and suggests that the current view on SRP-mediated protein targeting is incomplete. Structural basis for the molecular evolution of SRP-GTPase activation by protein.,Bange G, Kummerer N, Grudnik P, Lindner R, Petzold G, Kressler D, Hurt E, Wild K, Sinning I Nat Struct Mol Biol. 2011 Nov 6;18(12):1376-80. doi: 10.1038/nsmb.2141. PMID:22056770 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Flagellar biosynthetic protein Flagellar protein 3D structures GTP-binding protein 3D structures

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