7s0t

Structure of DNA polymerase zeta with mismatched DNA

spinqualitylabelsall models 7s0t, resolution 3.05Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7s0t is a 7 chain structure with sequence from Saccharomyces cerevisiae and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.05Å Ligands: CA, DCP, SF4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function DPOZ_YEAST Nonessential DNA polymerase. Required for DNA damage induced mutagenesis. Involved in DNA repair, mitochondrial DNA repair and translesion synthesis. Translesion synthesis in S.cerevisiae may use a specialized DNA polymerase that is not required for other DNA replicative processes. Has a role in the bypass of abasic (AP) sites. Highly inefficient in incorporating nucleotides opposite the AP site, but efficiently extends from nucleotides, particularly an A, inserted opposite the lesion. Publication Abstract from PubMed The B-family multi-subunit DNA polymerase zeta (Polzeta) is important for translesion DNA synthesis (TLS) during replication, due to its ability to extend synthesis past nucleotides opposite DNA lesions and mismatched base pairs. We present a cryo-EM structure of Saccharomyces cerevisiae Polzeta with an A:C mismatch at the primer terminus. The structure shows how the Polzeta active site responds to the mismatched duplex DNA distortion, including the loosening of key protein-DNA interactions and a fingers domain in an "open" conformation, while the incoming dCTP is still able to bind for the extension reaction. The structure of the mismatched DNA-Polzeta ternary complex reveals insights into mechanisms that either stall or favor continued DNA synthesis in eukaryotes. Cryo-EM structure of translesion DNA synthesis polymerase zeta with a base pair mismatch.,Malik R, Johnson RE, Prakash L, Prakash S, Ubarretxena-Belandia I, Aggarwal AK Nat Commun. 2022 Feb 25;13(1):1050. doi: 10.1038/s41467-022-28644-7. PMID:35217661 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also DNA polymerase 3D structures

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