1nx4: Difference between revisions

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[[Image:1nx4.~~gif~~|left|200px]]

[[Image:1nx4.png|left|200px]]

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~~'''~~The crystal structure of carbapenem synthase (CarC)~~'''~~

===The crystal structure of carbapenem synthase (CarC)===

~~==Overview==~~

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The ~~proposed biosynthetic pathway~~ to the ~~carbapenem antibiotics proceeds via epimerization~~/~~desaturation of a carbapenam in an unusual process catalyzed by an iron- and 2-oxoglutarate-dependent oxygenase, CarC~~. ~~Crystal structures of CarC complexed with Fe(II) and 2-oxoglutarate reveal it to be hexameric (space group C2221~~), ~~consistent with solution studies~~. ~~CarC monomers contain a double~~-~~stranded beta~~-helix core that supports ligands binding a single Fe(II) to which 2-oxoglutarate complexes in a bi-dentate manner. A structure was obtained with l-N-acetylproline acting as a substrate analogue. Quantum mechanical/molecular mechanical modeling studies with stereoisomers of carbapenams and carbapenems were used to investigate substrate binding. The combined work will stimulate further mechanistic studies and aid in the engineering of carbapenem biosynthesis.

The line below this paragraph, {{ABSTRACT_PUBMED_12611886}}, adds the Publication Abstract to the page

(as it appears on PubMed at http://www.pubmed.gov), where 12611886 is the PubMed ID number.

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==About this Structure==

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[[Category: Wilmouth, R C.]]

[[Category: Jelly roll]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 03~~:05:00 2008''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:48:12 2008''

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-[[Image:1nx4.gif|left|200px]]
+{{Seed}}
+[[Image:1nx4.png|left|200px]]
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 {{STRUCTURE_1nx4|  PDB=1nx4  |  SCENE=  }}
-'''The crystal structure of carbapenem synthase (CarC)'''
+===The crystal structure of carbapenem synthase (CarC)===
-==Overview==
+<!--
-The proposed biosynthetic pathway to the carbapenem antibiotics proceeds via epimerization/desaturation of a carbapenam in an unusual process catalyzed by an iron- and 2-oxoglutarate-dependent oxygenase, CarC. Crystal structures of CarC complexed with Fe(II) and 2-oxoglutarate reveal it to be hexameric (space group C2221), consistent with solution studies. CarC monomers contain a double-stranded beta-helix core that supports ligands binding a single Fe(II) to which 2-oxoglutarate complexes in a bi-dentate manner. A structure was obtained with l-N-acetylproline acting as a substrate analogue. Quantum mechanical/molecular mechanical modeling studies with stereoisomers of carbapenams and carbapenems were used to investigate substrate binding. The combined work will stimulate further mechanistic studies and aid in the engineering of carbapenem biosynthesis.
+The line below this paragraph, {{ABSTRACT_PUBMED_12611886}}, adds the Publication Abstract to the page
+(as it appears on PubMed at http://www.pubmed.gov), where 12611886 is the PubMed ID number.
+-->
+{{ABSTRACT_PUBMED_12611886}}
 ==About this Structure==
@@ Line 30: / Line 34: @@
 [[Category: Wilmouth, R C.]]
 [[Category: Jelly roll]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 03:05:00 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:48:12 2008''