4aco

Structure of the budding yeast Ndc10 N-terminal domain

spinqualitylabelsall models 4aco, resolution 1.89Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4aco is a 1 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.89Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function CBF3A_YEAST Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters. Publication Abstract from PubMed We have solved the X-ray structure of the amino-terminal half of the yeast kinetochore protein Ndc10 at 1.8 Angstrom resolution. This essential protein is a key constituent of the budding yeast centromere, and is essential for the recruitment of the centromeric nucleosome and establishment of the kinetochore. The fold of the protein shows unexpected similarities to the tyrosine recombinase/lambda-integrase family of proteins, most notably Cre, with some variation in the relative position of the sub-domains. This finding offers new insights into kinetochore evolution and the adaptation of a well-studied protein fold to a novel role. By comparison to tyrosine recombinases, and mutagenesis studies, we have been able to define some of the key DNA-binding motifs. The structure of the yeast kinetochore Ndc10 DNA-binding domain reveals an unexpected evolutionary relationship to tyrosine recombinases.,Perriches T, Singleton MR J Biol Chem. 2012 Jan 3. PMID:22215672 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Centromere protein 3D structure

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