3j01 | pdb_00003j01

Structure of the ribosome-SecYE complex in the membrane environment

spinqualitylabelsall models 3j01, resolution 7.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3j01 is a 33 chain structure with sequence from Ecol5, Ecoli and Escherichia coli dh1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3j00 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The ubiquitous SecY-Sec61 complex translocates nascent secretory proteins across cellular membranes and integrates membrane proteins into lipid bilayers. Several structures of mostly detergent-solubilized Sec complexes have been reported. Here we present a single-particle cryo-EM structure of the SecYEG complex in a membrane environment, bound to a translating ribosome, at subnanometer resolution. Using the SecYEG complex reconstituted in a so-called Nanodisc, we could trace the nascent polypeptide chain from the peptidyltransferase center into the membrane. The reconstruction allowed for the identification of ribosome-lipid interactions. The rRNA helix 59 (H59) directly contacts the lipid surface and appears to modulate the membrane in immediate vicinity to the proposed lateral gate of the protein-conducting channel (PCC). On the basis of our map and molecular dynamics simulations, we present a model of a signal anchor-gated PCC in the membrane. Cryo-EM structure of the ribosome-SecYE complex in the membrane environment.,Frauenfeld J, Gumbart J, Sluis EO, Funes S, Gartmann M, Beatrix B, Mielke T, Berninghausen O, Becker T, Schulten K, Beckmann R Nat Struct Mol Biol. 2011 May;18(5):614-21. Epub 2011 Apr 17. PMID:21499241 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Preprotein translocase Ribosome 3D structures

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