3iey | pdb_00003iey

Crystal Structure of the functional Nanoarchaeum equitans tRNA splicing endonuclease

spinqualitylabelsall models 3iey, resolution 2.11Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3iey is a 2 chain structure with sequence from Nanoarchaeum equitans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 3if0 Gene: endA, NEQ205 (Nanoarchaeum equitans), NEQ261 (Nanoarchaeum equitans) Activity: tRNA-intron endonuclease, with EC number 3.1.27.9 Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed The RNA splicing and processing endonuclease from Nanoarchaeum equitans (NEQ) belongs to the recently identified (alphabeta)(2) family of splicing endonucleases that require two different subunits for splicing activity. N. equitans splicing endonuclease comprises the catalytic subunit (NEQ205) and the structural subunit (NEQ261). Here, we report the crystal structure of the functional NEQ enzyme at 2.1 A containing both subunits, as well as that of the NEQ261 subunit alone at 2.2 A. The functional enzyme resembles previously known alpha(2) and alpha(4) endonucleases but forms a heterotetramer: a dimer of two heterodimers of the catalytic subunit (NEQ205) and the structural subunit (NEQ261). Surprisingly, NEQ261 alone forms a homodimer, similar to the previously known homodimer of the catalytic subunit. The homodimers of isolated subunits are inhibitory to heterodimerization as illustrated by a covalently linked catalytic homodimer that had no RNA cleavage activity upon mixing with the structural subunit. Detailed structural comparison reveals a more favorable hetero- than homodimerization interface, thereby suggesting a possible regulation mechanism of enzyme assembly through available subunits. Finally, the uniquely flexible active site of the NEQ endonuclease provides a possible explanation for its broader substrate specificity. Crystal structure and assembly of the functional Nanoarchaeum equitans tRNA splicing endonuclease.,Mitchell M, Xue S, Erdman R, Randau L, Soll D, Li H Nucleic Acids Res. 2009 Sep;37(17):5793-802. Epub 2009 Jul 3. PMID:19578064 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Endonuclease

Proteopedia Page Contributors and Editors (what is this?)

OCA