2x9t | pdb_00002x9t

STRUCTURE OF THE 70S RIBOSOME BOUND TO RELEASE FACTOR 2 AND A SUBSTRATE ANALOG PROVIDES INSIGHTS INTO CATALYSIS OF PEPTIDE RELEASE

spinqualitylabelsall models 2x9t, resolution 3.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2x9t is a 25 chain structure with sequence from Escherichia coli and Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: MG, ZN NonStd Res: 8AN, PHA Related: 2x9r, 2x9s, 2x9u, 1ibk, 1ibl, 2wdk, 2uuc, 2j02, 1j5e, 2wrq, 2b9o, 1hnx, 2j00, 2wh3, 2uub, 1vov, 1emi, 1pnx, 2v46, 2vqf, 2wh1, 1n32, 2wrn, 2f4v, 1twt, 1qzc, 2uxd, 1fka, 2uxc, 1n36, 1fjg, 1hnw, 1l1u, 1hr0, 2v48, 2b64, 1xmq, 2b9m, 1pn8, 1xnr, 2wdg, 1hnz, 1dv4, 1rss, 2wdh, 1jgq, 1i94, 1i96, 2uu9, 1pn7, 1eg0, 1ibm, 2uxb, 2vqe, 1g1x, 2uua, 1i95, 1xnq, 1qd7, 1gix, 1jgo, 1jgp, 1n34, 1n33, 1pns, 1yl4, 2wdm, 1xmo, 1i97 Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed We report the crystal structure of release factor 2 bound to ribosome with an aminoacyl tRNA substrate analog at the ribosomal P site, at 3.1 A resolution. The structure shows that upon stop-codon recognition, the universally conserved GGQ motif packs tightly into the peptidyl transferase center. Nucleotide A2602 of 23S rRNA, implicated in peptide release, packs with the GGQ motif in release factor 2. The ribose of A76 of the peptidyl-tRNA adopts the C2'-endo conformation, and the 2' hydroxyl of A76 is within hydrogen-bond distance of the 2' hydroxyl of A2451. The structure suggests how a catalytic water can be coordinated in the peptidyl transferase center and, together with previous biochemical and computational data, suggests a model for how the ester bond between the peptidyl tRNA and the nascent peptide is hydrolyzed. Structure of the 70S ribosome bound to release factor 2 and a substrate analog provides insights into catalysis of peptide release.,Jin H, Kelley AC, Loakes D, Ramakrishnan V Proc Natl Acad Sci U S A. 2010 Apr 26. PMID:20421507 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Ribosomal protein THX Ribosome 3D structures

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