2y12 | pdb_00002y12

THE CRYSTAL STRUCTURE OF EF-TU AND G24A-TRNA-TRP BOUND TO A NEAR-COGNATE CODON ON THE 70S RIBOSOME

spinqualitylabelsall models 2y12, resolution 3.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2y12 is a 26 chain structure with sequence from Escherichia coli and Thermus thermophilus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GDP, KIR, ZN NonStd Res: 4SU, 5MU, 7MG, H2U, MIA, OMC, PSU Related: 2y0y, 2y0z, 2y13, 1ibk, 1ibl, 2uuc, 2wdk, 2y0u, 2j02, 1j5e, 2wrq, 1hnx, 2j00, 2b9o, 2x9t, 2uub, 2wh3, 1vov, 1emi, 2xqd, 1pnx, 2v46, 2vqf, 2wh1, 1n32, 2wrn, 2jl5, 2f4v, 1twt, 1qzc, 2uxd, 1fka, 1fjg, 1n36, 2uxc, 1hnw, 1l1u, 1hr0, 2v48, 2xuy, 2b64, 1xmq, 2b9m, 1pn8, 2wdg, 1xnr, 1dv4, 1hnz, 1rss, 2wdh, 1jgq, 1i94, 2xsy, 1i96, 2uu9, 1pn7, 1eg0, 1ibm, 2uxb, 2vqe, 1g1x, 1ha3, 1i95, 2uua, 1xnq, 2xfz, 1qd7, 1gix, 1jgo, 1jgp, 2xg1, 2c78, 2x9r, 1n34, 2y0w, 1n33, 2jl7, 1pns, 1yl4, 2wdm, 1xmo, 1i97, 2y17, 2y19, 2y16, 2y10, 2y15, 2y11, 2y14, 2y18 Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed The ribosome converts genetic information into protein by selecting aminoacyl tRNAs whose anticodons base-pair to an mRNA codon. Mutations in the tRNA body can perturb this process and affect fidelity. The Hirsh suppressor is a well-studied tRNA(Trp) harboring a G24A mutation that allows readthrough of UGA stop codons. Here we present crystal structures of the 70S ribosome complexed with EF-Tu and aminoacyl tRNA (native tRNA(Trp), G24A tRNA(Trp) or the miscoding A9C tRNA(Trp)) bound to cognate UGG or near-cognate UGA codons, determined at 3.2-A resolution. The A9C and G24A mutations lead to miscoding by facilitating the distortion of tRNA required for decoding. A9C accomplishes this by increasing tRNA flexibility, whereas G24A allows the formation of an additional hydrogen bond that stabilizes the distortion. Our results also suggest that each native tRNA will adopt a unique conformation when delivered to the ribosome that allows accurate decoding. How mutations in tRNA distant from the anticodon affect the fidelity of decoding.,Schmeing TM, Voorhees RM, Kelley AC, Ramakrishnan V Nat Struct Mol Biol. 2011 Apr;18(4):432-6. Epub 2011 Mar 6. PMID:21378964 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Elongation factor Ribosomal protein THX Ribosome 3D structures

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