4npl | pdb_00004npl

Crystal structure of Zebrafish ALKBH5 in complex with alpha-ketoglutarate

spinqualitylabelsall models 4npl, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4npl is a 2 chain structure with sequence from Brachidanio rerio. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: AKG, MN Related: 4npm Gene: alkbh5 (Brachidanio rerio) Resources: FirstGlance, OCA, RCSB, PDBsum Function [ALKB5_DANRE] Dioxygenase that demethylates RNA by oxidative demethylation: specifically demethylates N(6)-methyladenosine (m(6)A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Requires molecular oxygen, alpha-ketoglutarate and iron (By similarity). Publication Abstract from PubMed ALKBH5, a member of AlkB family proteins, has been reported as a mammalian N(6)-methyladenosine (m(6)A) RNA demethylase. Here we report the crystal structure of zebrafish ALKBH5 (fALKBH5) with the resolution of 1.65A. Structural superimposition shows that fALKBH5 is comprised of a conserved jelly-roll motif. However, it possesses a loop that interferes potential binding of a duplex nucleic acid substrate, suggesting an important role in substrate selection. In addition, several active site residues are different between the two known m(6)A RNA demethylases, ALKBH5 and FTO, which may result in their slightly different pathways of m(6)A demethylation. Crystal structure of the RNA demethylase ALKBH5 from zebrafish.,Chen W, Zhang L, Zheng G, Fu Y, Ji Q, Liu F, Chen H, He C FEBS Lett. 2014 Mar 18;588(6):892-8. doi: 10.1016/j.febslet.2014.02.021. Epub, 2014 Feb 20. PMID:24561204 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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