1p58 | pdb_00001p58
Complex Organization of Dengue Virus Membrane Proteins as Revealed by 9.5 Angstrom Cryo-EM reconstruction
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| 1p58 | |||||||||||||
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| Related: | 1K4R, 1SVB, 1JCH
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Overview
Improved technology for reconstructing cryo-electron microscopy (cryo-EM) images has now made it possible to determine secondary structural features of membrane proteins in enveloped viruses. The structure of mature dengue virus particles was determined to a resolution of 9.5 A by cryo-EM and image reconstruction techniques, establishing the secondary structural disposition of the 180 envelope (E) and 180 membrane (M) proteins in the lipid envelope. The alpha-helical 'stem' regions of the E molecules, as well as part of the N-terminal section of the M proteins, are buried in the outer leaflet of the viral membrane. The 'anchor' regions of E and the M proteins each form antiparallel E-E and M-M transmembrane alpha-helices, leaving their C termini on the exterior of the viral membrane, consistent with the predicted topology of the unprocessed polyprotein. This is one of only a few determinations of the disposition of transmembrane proteins in situ and shows that the nucleocapsid core and envelope proteins do not have a direct interaction in the mature virus.
About this Structure
1P58 is a Protein complex structure of sequences from Dengue virus 2. Full crystallographic information is available from OCA.
Reference
Visualization of membrane protein domains by cryo-electron microscopy of dengue virus., Zhang W, Chipman PR, Corver J, Johnson PR, Zhang Y, Mukhopadhyay S, Baker TS, Strauss JH, Rossmann MG, Kuhn RJ, Nat Struct Biol. 2003 Nov;10(11):907-12. Epub 2003 Oct 5. PMID:14528291
Page seeded by OCA on Sun Mar 30 22:55:01 2008
Proteopedia Page Contributors and Editors (what is this?)
- Pages with broken file links
- Dengue virus 2
- Protein complex
- Baker, T S.
- Chipman, P R.
- Corver, J.
- Johnson, P R.
- Kuhn, R J.
- Mukhopadhyay, S.
- Rossmann, M G.
- Strauss, J H.
- Zhang, W.
- Zhang, Y.
- Cryo-em
- Dengue virus
- Flaviviridae
- Flavivirus
- Glycoprotein e from tick-borne encephalitis virus
- Icosahedral virus
- Membrane protein m
