3jb9 | pdb_00003jb9
Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution
Structural highlights
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Function[RU2B_SCHPO] Involved in pre-mRNA splicing. This protein is associated with snRNP U2. It binds stem loop IV of U2 snRNA (By similarity). [SLT11_SCHPO] Facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome. Binds to RNA (By similarity). Involved in pre-mRNA splicing. [SMD2_SCHPO] Involved in pre-mRNA splicing. Binds snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site (By similarity). [CWC15_SCHPO] Involved in pre-mRNA splicing. [SN114_SCHPO] Component of the U5 snRNP complex required for pre-mRNA splicing. Binds GTP. [SPP42_SCHPO] Involved in pre-mRNA splicing. May be involved in endoplasmic reticulum-associated protein degradation (ERAD) and required for growth at low and high temperatures (By similarity). Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U5. Has a role in branch site-3' splice site selection. Associates with the branch site-3' splice 3'-exon region.[1] [PRP19_SCHPO] Probable ubiquitin-protein ligase involved in pre-mRNA splicing where it associates with cdc5 and the other cwf proteins as part of the spliceosome. May also function in DNA repair.[UniProtKB:P32523] [CWF11_SCHPO] Involved in mRNA splicing where it associates with cdc5 and the other cwf proteins as part of the spliceosome. [SPF27_SCHPO] Involved in mRNA splicing. [PRP17_SCHPO] Functions in the second step of pre-mRNA splicing. Involved in splicing intron which are longer than 200 nucleotides.[2] [CWC2_SCHPO] Involved in the first step of pre-mRNA splicing. Required for cell growth and cell cycle control. Plays a role in the levels of the U1, U4, U5 and U6 snRNAs and the maintenance of the U4/U6 snRNA complex. May provide the link between the "nineteen complex" NTC spliceosome protein complex and the spliceosome through the U6 snRNA. Associates predominantly with U6 snRNAs in assembled active spliceosomes. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle (By similarity). Involved in pre-mRNA splicing.[3] [CWF14_SCHPO] Involved in mRNA splicing where it associates with cdc5 and the other cwf proteins as part of the spliceosome. [RUXG_SCHPO] Involved in pre-mRNA splicing. Binds snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site (By similarity). [RU2A_SCHPO] Involved in pre-mRNA splicing. This protein is associated with sn-RNP U2. It helps the A' protein to bind stem loop IV of U2 snRNA (By similarity). [CWF19_SCHPO] Involved in mRNA splicing where it associates with cdc5 and the other cwf proteins as part of the spliceosome. Involved in mRNA splicing. [PRP45_SCHPO] Involved in pre-mRNA splicing. [PPIL1_SCHPO] PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity). [SMD1_SCHPO] Involved in pre-mRNA splicing. Binds snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site (By similarity). [RUXF_SCHPO] Involved in pre-mRNA splicing. Binds snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site (By similarity). [CWF17_SCHPO] Involved in mRNA splicing where it associates with cdc5 and the other cwf proteins as part of the spliceosome. [RUXE_SCHPO] Involved in pre-mRNA splicing. Binds and is required for the stability of snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site. Involved in cap modification (By similarity).[UniProtKB:Q12330] [SMD3_SCHPO] Involved in pre-mRNA splicing. Binds snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site (By similarity). [CEF1_SCHPO] Involved in mRNA splicing and cell cycle control.[4] [5] [RSMB_SCHPO] Involved in pre-mRNA splicing. Binds snRNA U1, U2, U4 and U5 which contain a highly conserved structural motif called the Sm binding site (By similarity). Publication Abstract from PubMedSplicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs), NTC (nineteen complex), NTC-related proteins (NTR), and a number of associated enzymes and cofactors. Here, we report the three-dimensional structure of a Schizosaccharomyces pombe spliceosome at 3.6-angstrom resolution, revealed by means of single-particle cryogenic electron microscopy. This spliceosome contains U2 and U5 snRNPs, NTC, NTR, U6 small nuclear RNA, and an RNA intron lariat. The atomic model includes 10,574 amino acids from 37 proteins and four RNA molecules, with a combined molecular mass of approximately 1.3 megadaltons. Spp42 (Prp8 in Saccharomyces cerevisiae), the key protein component of the U5 snRNP, forms a central scaffold and anchors the catalytic center. Both the morphology and the placement of protein components appear to have evolved to facilitate the dynamic process of pre-mRNA splicing. Our near-atomic-resolution structure of a central spliceosome provides a molecular framework for mechanistic understanding of pre-mRNA splicing. Structure of a yeast spliceosome at 3.6-angstrom resolution.,Yan C, Hang J, Wan R, Huang M, Wong CC, Shi Y Science. 2015 Sep 11;349(6253):1182-91. doi: 10.1126/science.aac7629. Epub 2015, Aug 20. PMID:26292707[6] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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