Vinculin

spinqualitylabelsall models Chicken full-length metavinculin, 1st6 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Function Vinculins (VCLs) are involved in adhesion by linking integrin molecules to the actin cytoskeleton. Its head domain (Vd1) can bind to talin or to alpha-actinin at their respective VCL Binding Sites (VBS). The protein raver1 RNA Recognition Motif (RRM) forms a complex with VCL or m-VCL. Metavinculin (m-VCL) is a splice version of VCL containing an extra ca. 70 amino acids in the C-terminal domain. Relevance Loss of VCL could be used as a prognostic factor for colorectal cancer se it promotes metastasis. Disease Mutation in m-VCL can yield cardiomyopathic phenotype. Structural highlights Vinculin Autoinhibition is achieved through a high affinity intramolecular interaction between tail (orange) and head (aqua) domains (1st6). Energetically, I997 is key to maintaining this autoinhibition.

3D Structures of Vinculin

Updated on 05-October-2018

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• Vinculin
  • talin – hVCL - human
    
  • alpha-actinin – hVCL tail domain
    
  • 1st6 - hVCL tail domain + raver1 RRM
    
  • 4pr9 - hVCL tail domain + lipid
    
  • 3jbi - hVCL tail domain + actin – Cryo-EM
    
  • 3h2u – hVCL Vd1 + raver1 RRM
    
  • 2ibf, 2hsq, 2gww - hVCL Vd1 + SfVCL binding sites from Shigella flexneri
    
  • 1ydi - hVCL Vd1+hActinin VBS
    
  • 1t01 - cVCL Vd1+mTalin VBS – chicken
    
  • 1syq, 1rkc, 1rke – hVCL Vd1+hTalin VBS
    
  • 3zdl – cVCL Vd1 + amyloid β precursor protein N terminal
    
• Metavinculin
  • 3myi – m-VCL tail domain
    
  • 5l0f, 5l0i, 5l0j - hm-VCL tail domain (mutant)
    
  • 5l0c, 5l0d - hm-VCL tail domain + lipid
    
  • 5l0g, 5l0h - hm-VCL tail domain (mutant) + lipid
    
  • 3jbk – hm-VCL tail domain + actin – Cryo-EM
    
  • 3rf3 - hm-VCL + invasin IPAA
    
  • 3s90 - hm-VCL head domain + mTalin-1 peptide
    
  • 4dj9 - hm-VCL head domain + hTalin-1 peptide
    
  • 3tj5 - hm-VCL head domain + Sca-family protein peptide
    
  • 3tj6 - hm-VCL head domain + protein Ps 120 peptide
    
  • 4ehp - hm-VCL head domain + catenin α-1 residues 277-382
    
  • 3vf0 – hm-VCL residues 856-1134 + ribonucleoprotein PTB-binding
    
  • 2gdc – cm-VCL Vd1+SfInvasin C-terminal
    
  • 1xwj - cm-VCL Vd1+cTalin VBS3
    
  • 1zvz, 1zw2, 1zw3, 1u6h - cm-VCL Vd1+cTalin rod
    
  • 1st6 – cm-VCL
    
  • 4e17, 4e18 - cm-VCL Vd1 + catenin α-1 VCL-binding domain

References

• Created with the participation of Susan Craig.

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Alexander Berchansky, Jaime Prilusky, Michal Harel